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Tick salivary cystatin sialostatin L2 suppresses IFN responses in mouse dendritic cells
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SYSNO ASEP 0453400 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Tick salivary cystatin sialostatin L2 suppresses IFN responses in mouse dendritic cells Author(s) Lieskovská, Jaroslava (BC-A) RID
Páleníková, Jana (BC-A)
Širmarová, J. (CZ)
Elsterová, Jana (BC-A) ORCID
Kotsyfakis, Michalis (BC-A) RID, ORCID
Chagas, A. C. (US)
Calvo, E. (US)
Růžek, Daniel (BC-A) RID, ORCID
Kopecký, Jan (BC-A) RIDSource Title Parasite immunology. - : Wiley - ISSN 0141-9838
Roč. 37, č. 2 (2015), s. 70-78Number of pages 9 s. Publication form Print - P Language eng - English Country GB - United Kingdom Keywords Tick ; Dendritic cells ; Interferon ; Cystatin Subject RIV EC - Immunology R&D Projects GAP302/12/2208 GA ČR - Czech Science Foundation (CSF) Institutional support BC-A - RVO:60077344 UT WOS 000348718100002 DOI 10.1111/pim.12162 Annotation Type I interferon (IFN), mainly produced by dendritic cells (DCs), is critical in the host defence against tick-transmitted pathogens. Here, we report that salivary cysteine protease inhibitor from the hard tick Ixodes scapularis, sialostatin L2, affects IFN- mediated immune reactions in mouse dendritic cells. Following IFN receptor ligation, the Janus activated kinases/signal transducer and activator of transcription (JAK/STAT) pathway is activated. We show that sialostatin L2 attenuates phosphorylation of STATs in spleen dendritic cells upon addition of recombinant IFN-. LPS-stimulated dendritic cells release IFN- which in turn leads to the induction of IFN-stimulated genes (ISG) through JAK/STAT pathway activation. The induction of two ISG, interferon regulatory factor 7 (IRF-7) and IP-10, was suppressed by sialostatin L2 in LPS-stimulated dendritic cells. Finally, the interference of sialostatin L2 with IFN action led to the enhanced replication of tick-borne encephalitis virus in DC. In summary, we present here that tick salivary cystatin negatively affects IFN- responses which may consequently increase the pathogen load after transmission via tick saliva. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2016
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