Pseudomonas coleopterorum sp nov., a cellulase-producing bacterium isolated from the bark beetle Hylesinus fraxini

Menéndez, E.; Ramírez-Bahena, M.H.; Fabryová, A.; Igual, J.M.; Benada, Oldřich

doi:https://dx.doi.org/10.1099/ijs.0.000344

Number of the records: 1

Pseudomonas coleopterorum sp nov., a cellulase-producing bacterium isolated from the bark beetle Hylesinus fraxini

1.

SYSNO ASEP	0452560
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Pseudomonas coleopterorum sp nov., a cellulase-producing bacterium isolated from the bark beetle Hylesinus fraxini
Author(s)	Menéndez, E. (ES) Ramírez-Bahena, M.H. (ES) Fabryová, A. (CZ) Igual, J.M. (CZ) Benada, Oldřich (MBU-M)_{ORCID, RID}
Source Title	International Journal of Systematic and Evolutionary Microbiology. - : Microbiology Society - ISSN 1466-5026 Roč. 65, SEP 2015 (2015), s. 2852-2858
Number of pages	7 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	CURCULIONIDAE SCOLYTINAE ; NUCLEOTIDE-SEQUENCES ; DENDROCTONUS-RHIZOPHAGUS
Subject RIV	EE - Microbiology, Virology
R&D Projects	EE2.3.30.0003 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	MBU-M - RVO:61388971
UT WOS	000364108400011
DOI	10.1099/ijs.0.000344
Annotation	We isolated a strain coded Esc2Am(T) during a study focused on the microbial diversity of adult specimens of the bark beetle Hylesinus fraxini. Its 16S rRNA gene sequence had 99.4 % similarity with respect to its closest relative, Pseudomonas rhizosphaerae IH5(T). The analysis of partial sequences of the housekeeping genes rpoB, rpoD and gyrB confirmed that strain Esc2Am(T) formed a cluster with P. rhizosphaerae IH5(T) clearly separated from the remaining species of the genus Pseudomonas. Strain Esc2Am(T) had polar flagella and could grow at temperatures from 4 degrees C to 30 degrees C. The respiratory quinone was 09 and the main fatty acids were C-16 : 0, C-18 (:) (1)omega 7c and/or C-18 (:) (1)omega 6c in summed feature 8 and C-16 (:) (1)omega 7c and/or C-18 : 1 omega 6c in summed feature 3. DNA DNA hybridization results showed 51 % relatedness with respect to P. rhizosphaerae IH5(T). Oxidase, catalase and urease-positive, the arginine dihydrolase system was present but nitrate reduction and beta-galactosidase production were negative. Aesculin hydrolysis was positive. Based on the results from the genotypic, phenotypic and chemotaxonomic analyses, we propose the classification of strain Esc2Am(T) as representing a novel species of the genus Pseudomonas, for which we propose the name Pseudomonas coleopterorum sp. nov. The type strain is Esc2Am(T) (=LMG 28558(T)=CECT 8695(T)).
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2016

Number of the records: 1