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Binding of palladium (II) 5, 10, 15, 20-tetrakis (4-sulfonatophenyl) porphyrin to a lectin for photosensitizer targeted delivery
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SYSNO ASEP 0450838 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Binding of palladium (II) 5, 10, 15, 20-tetrakis (4-sulfonatophenyl) porphyrin to a lectin for photosensitizer targeted delivery Author(s) Bogoeva, V. (BG)
Petrova, L. (BG)
Kubát, Pavel (UFCH-W) RID, ORCID, SAISource Title Journal of Photochemistry and Photobiology. B - Biology Section. - : Elsevier - ISSN 1011-1344
Roč. 153, DEC 2015 (2015), s. 276-280Number of pages 5 s. Language eng - English Country CH - Switzerland Keywords palladium porphyrin ; concavalin A ; fluorescence Subject RIV CF - Physical ; Theoretical Chemistry R&D Projects GA13-12496S GA ČR - Czech Science Foundation (CSF) Institutional support UFCH-W - RVO:61388955 UT WOS 000366765400033 EID SCOPUS 84945181402 DOI 10.1016/j.jphotobiol.2015.10.010 Annotation This paper describes the interaction between a photosensitizer, palladium 5, 10, 15, 20-tetrakis (4-sulfonatophenyl) porphyrin (PdTPPS), and Concanavalin A (Con A), a potential drug carrier that selectively agglutinates tumor cells. Steady-state fluorescence showed protein conformational rearrangements upon PdTPPS binding. The dissociation constant (KD) of 0.31±0.13 μM showed high-affinity binding of the photosensitizer to the protein and more than one class of binding site with positive cooperativity, as confirmed by the Hill slope. Transient absorption spectroscopy revealed two distinguishable porphyrin forms of the bound and the free triplet states of PdTPPS. The kinetics of the photosensitizer triplets in air-saturated PBS buffer is monoexponential with a lifetime of 1.6μs, while the kinetics of the complex PdTPPS-Con A is biexponential with a lifetime of 18μs, which is one order of magnitude lower than that of the free PdTPPS. The quenching of the triplet states of the photosensitizer-Con A complex led to the formation of singlet oxygen, O2(1Δg). PdTPPS remains a good producer of O2(1Δg) in the complex and may find potential utility in the application of photodynamic therapy (PDT) for cancer. Workplace J. Heyrovsky Institute of Physical Chemistry Contact Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Year of Publishing 2016
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