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Monitoring of Multilayered Bacterial Biofilm Morphology by Cryo-SEM for Raman Spectroscopy Measurements
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SYSNO ASEP 0450101 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Monitoring of Multilayered Bacterial Biofilm Morphology by Cryo-SEM for Raman Spectroscopy Measurements Author(s) Hrubanová, Kamila (UPT-D) RID, SAI, ORCID
Bernatová, Silvie (UPT-D) RID, SAI
Samek, Ota (UPT-D) RID, ORCID, SAI
Šerý, Mojmír (UPT-D) RID, SAI
Zemánek, Pavel (UPT-D) RID, SAI, ORCID
Nebesářová, Jana (BC-A) RID, ORCID
Růžička, F. (CZ)
Krzyžánek, Vladislav (UPT-D) RID, ORCID, SAINumber of authors 8 Source Title Microscopy and Microanalysis. - : Cambridge University Press - ISSN 1431-9276
Roč. 21, S3 (2015), s. 187-188Number of pages 2 s. Publication form Print - P Language eng - English Country US - United States Keywords multilayered bacterial biofilm ; morphology by Cryo-SEM ; Raman spectroscopy Subject RIV JA - Electronics ; Optoelectronics, Electrical Engineering R&D Projects LO1212 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA14-20012S GA ČR - Czech Science Foundation (CSF) Institutional support UPT-D - RVO:68081731 ; BC-A - RVO:60077344 Annotation Staphylococcus epidermidis has been recently recognized as an important cause of serious biofilm infections associated with implanted medical devices. In presented work the multi-layered biofilms formed by these microorganisms were observed by scanning electron microscope (SEM), in particular with using freeze-fracturing technique. The freeze-fracture technique consists of physically breaking apart (fracturing) a rapidly frozen biological sample; structural detail exposed by the fracture plane is then visualized by metal deposition. An optional step, involving vacuum sublimation of ice, may be carried out after fracturing. Freeze fracture is unique among electron microscopic techniques in providing planar views of the internal organization of membranes or biofilms. Deep etching of ultra-rapidly frozen samples permits visualization of the surface structure of cells and their components. Our sample was fractured after rapid-freezing into liquid nitrogen, the air-water was removed by short freeze-drying at Alto 2500 chamber (Gatan), sputtered by 5 nm Pt-Pd and finally imaged at low temperature in the field emission SEM JSM 7401F (JEOL). Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2016
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