TALENs: Methods and Protocols

0448235 - BC 2016 US eng M - Monography Chapter
Takasu, Y. - Tamura, T. - Goldsmith, M. - Žurovec, Michal
Targated mutagensis in Bombyx mori Using TALENs.
TALENs: Methods and Protocols. Vol. 1338. New York: Springer New York, 2016 - (Kühn, R.; Wurst, W.; Wefers, B.), s. 127-142. ISBN 978-1-4939-2931-3
Institutional support: RVO:60077344
Keywords : silkworm * pBlue-TAL * engineered nucleases
Subject RIV: EB - Genetics ; Molecular Biology

Bombyx mori is a valuable model organism of high economic importance. Its genome sequence is available, as well as basic genetic and molecular genetic tools and markers. The introduction of genome editing methods based on engineered nucleases enables precise manipulations with genomic DNA, including targeted DNA deletions, insertions, or replacements in the genome allowing gene analysis and various applications. We describe here the use of TALENs which have a simple modular design of their DNA-binding domains, are easy to prepare and proved to be efficient in targeting of a wide range of cleavage sites. Our procedure often allows the production of individuals carrying homozygous mutations as early as in the G1 generation.
Permanent Link: http://hdl.handle.net/11104/0257761