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Evalution of the diet of a carabid predator using fluorescent marking of prey
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SYSNO ASEP 0446125 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Evalution of the diet of a carabid predator using fluorescent marking of prey Author(s) Okrouhlík, Jan (BC-A)
Foltan, Pavel (BC-A)Number of authors 2 Source Title European Journal of Entomology. - : Biologické centrum AV ČR, v. v. i. - ISSN 1210-5759
Roč. 112, č. 3 (2015), s. 477-485Number of pages 9 s. Language eng - English Country CZ - Czech Republic Keywords food web ; prey marking ; fluorescent dye Subject RIV EH - Ecology, Behaviour Institutional support BC-A - RVO:60077344 UT WOS 000360715400011 EID SCOPUS 84942863783 DOI 10.14411/eje.2015.054 Annotation We present a UV-fluorescent dye-based prey marking technique, using three different dyes, with later detection of the dyes in the guts of predators using a micro-plate fluorescent reader. By using this new method of detecting dyes based on their different excitation and emission characteristics it is possible to simultaneously screen 96 samples for multiple fluorescent-markers in less than 30 minutes. The marking is stable, cheap, non-toxic and had no effect on the choice of the carabid predator Pterostichus melanarius between marked and unmarked fly larvae (Musca domestica). Different fluorescent dyes provide variable detection intervals up to 100% detectability at 96 h post-ingestion by this predator. The simple marking, extraction and detection methods presented could be used in food web research to map individual trophic links and predator preference for different types of prey. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2016 Electronic address http://www.eje.cz/pdfs/eje/2015/03/11.pdf
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