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Role of c-Myb in chondrogenesis
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SYSNO ASEP 0445254 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Role of c-Myb in chondrogenesis Author(s) Oralová, Veronika (UZFG-Y) RID, ORCID
Matalová, Eva (UZFG-Y) RID
Janečková, Eva (UZFG-Y)
Drobná Krejčí, E. (CZ)
Knopfová, L. (CZ)
Šnajdr, P. (CZ)
Tucker, A. S. (GB)
Veselá, I. (CZ)
Šmarda, J. (CZ)
Buchtová, Marcela (UZFG-Y) RID, ORCIDSource Title Bone. - : Elsevier - ISSN 8756-3282
Roč. 76, č. 1 (2015), s. 97-106Number of pages 10 s. Publication form Print - P Language eng - English Country US - United States Keywords micromass cultures ; mouse limbs ; endochondral bone Subject RIV EA - Cell Biology R&D Projects GCP302/12/J059 GA ČR - Czech Science Foundation (CSF) GB14-37368G GA ČR - Czech Science Foundation (CSF) Institutional support UZFG-Y - RVO:67985904 UT WOS 000355718100012 EID SCOPUS 84927656029 DOI 10.1016/j.bone.2015.02.031 Annotation The Myb locus encodes the c-Myb transcription factor involved in controlling abroad variety of cellular processes. Recently, it has been shown that c-Myb may play a specific role in hard tissue formation; however, all of these results were gathered from an analysis of intramembranous ossification. To investigate a possible role of c-Myb in endochondral ossification, we carried out our study on the long bones of mouse limbs during embryonic development. Firstly, the c-myb expression pattern was analyzed by in situ hybridization during endochondral ossification of long bones. c-myb positive areas were found in proliferating as well as hypertrophic zones of the growth plate. At early embryonic stages, localized expression was also observed in the perichondrium and interdigital areas. The c-Myb protein was found in proliferating chondrocytes and in the perichondrium of the forelimb bones (E14.5-E17.5). Furthermore, protein was detected in pre-hypertrophic as well as hypertrophic chondrocytes. Gain-of-function and loss-of-function approaches were used to test the effect of altered c-myb expression on chondrogenesis in micromass cultures established from forelimb buds of mouse embryos. A loss-of-function approach using c-myb specific siRNA decreased nodule formation, as well as downregulated the level of Sox9 expression, a major marker of chondrogenesis. Transient c-myb overexpression markedly increased the formation of cartilage nodules and the production of extracellular matrix as detected by intense staining with Alcian blue. Moreover, the expression of early chondrogenic genes such as Sox9, Col2a1 and activity of a Col2-LUC reporter were increased in the cells overexpressing c-myb while late chondrogenic markers such as Col10a1 and Mmp13 were not significantly changed or were downregulated. Taken together, the results of this study demonstrate that the c-Myb transcription factor is involved in the regulation and promotion of endochondral bone formation. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2016
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