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Transferable antibiotic resistance plasmids from biogas plant digestates often belong to the IncP-1 epsilon subgroup
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SYSNO ASEP 0444547 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Transferable antibiotic resistance plasmids from biogas plant digestates often belong to the IncP-1 epsilon subgroup Author(s) Wolters, B. (DE)
Kyselková, Martina (BC-A) RID
Krögerrecklenfort, E. (DE)
Kreuzig, R. (DE)
Smalla, K. (DE)Source Title Frontiers in Microbiology. - : Frontiers Research Foundation - ISSN 1664-302X
Roč. 5, January (2015), Article 765Number of pages 11 s. Language eng - English Country CH - Switzerland Keywords IncP-1 epsilon plasmid ; class 1 integrons ; biogas plant digestate ; antibiotic resistance ; exogenous plasmid isolation Subject RIV EE - Microbiology, Virology R&D Projects EE2.3.30.0032 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support BC-A - RVO:60077344 UT WOS 000348859400001 EID SCOPUS 84927518366 DOI 10.3389/fmicb.2014.00765 Annotation Manure is known to contain residues of antibiotics administered to farm animals as well as bacteria carrying antibiotic resistance genes (ARGs). These genes are often located on mobile genetic elements. In biogas plants (BGPs), organic substrates such as manure and plant material are mixed and fermented in order to provide energy, and resulting digestates are used for soil fertilization. The fate of plasmid carrying bacteria from manure during the fermentation process is unknown. The present study focused on transferable antibiotic resistance plasmids from digestates of seven BGPs, using manure as a co-substrate, and their phenotypic and genotypic characterization. Plasmids conferring resistance to either tetracycline or sulfadiazine were captured by means of exogenous plasmid isolation from digestates into Pseudomonas putida KT2442 and Escherichia coli CV601 recipients, at transfer frequencies ranging from 10(-5) to 10(-7). Transconjugants (n = 101) were screened by PCR-Southern blot hybridization and real-time PCR for the presence of IncP-1, IncP-1 epsilon, IncW, IncN, IncP-7, IncP-9, LowGC, and IncQ plasmids. While 61 plasmids remained unassigned, 40 plasmids belonged to the IncP-1 epsilon subgroup. All these IncP-1 epsilon plasmids were shown to harbor the genes tet(A), sul1, qacE Delta 1, intl1, and integron gene cassette amplicons of different size. Further analysis of 16 representative IncP-1 epsilon plasmids showed that they conferred six different multiple antibiotic resistance patterns and their diversity seemed to be driven by the gene cassette arrays. IncP-1 epsilon plasmids displaying similar restriction and antibiotic resistance patterns were captured from different BGPs, suggesting that they may be typical of this environment. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2016
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