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TAL effectors: tools for DNA Targeting
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SYSNO ASEP 0442505 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title TAL effectors: tools for DNA Targeting Author(s) Jankele, R. (CZ)
Svoboda, Petr (UMG-J) RIDSource Title Briefings in Functional Genomics - ISSN 2041-2649
Roč. 13, č. 5 (2014), s. 409-419Number of pages 11 s. Language eng - English Country GB - United Kingdom Keywords TALE ; TALEN ; DNA editing Subject RIV EB - Genetics ; Molecular Biology R&D Projects GBP305/12/G034 GA ČR - Czech Science Foundation (CSF) Institutional support UMG-J - RVO:68378050 UT WOS 000342911500009 DOI 10.1093/bfgp/elu013 Annotation Xanthomonas phytopathogenic bacteria produce unique transcription activator-like effector (TALE) proteins that recognize and activate specific plant promoters through a set of tandem repeats. A unique TALE-DNA-binding code uses two polymorphic amino acids in each repeat to mediate recognition of specific nucleotides. The order of repeats determines effector's specificity toward the cognate nucleotide sequence of the sense DNA strand. Artificially designed TALE-DNA-binding domains fused to nuclease or activation and repressor domains provide an outstanding toolbox for targeted gene editing and gene regulation in research, biotechnology and gene therapy. Gene editing with custom-designed TALE nucleases (TALENs) extends the repertoire of targeted genome modifications across a broad spectrum of organisms ranging from plants and insect to mammals. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2015
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