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In vivo effects of focused shock waves on tumor tissue visualized by fluorescence staining techniques
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SYSNO ASEP 0441045 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title In vivo effects of focused shock waves on tumor tissue visualized by fluorescence staining techniques Author(s) Lukeš, Petr (UFP-V) RID, ORCID
Zeman, J. (CZ)
Horák, Vratislav (UZFG-Y) RID, ORCID
Hoffer, Petr (UFP-V) RID
Poučková, P. (CZ)
Holubová, Monika (UZFG-Y)
Hosseini, S.H.R. (CA)
Akiyama, H. (JP)
Šunka, Pavel (UFP-V) RID
Beneš, J. (CZ)Source Title Bioelectrochemistry. - : Elsevier - ISSN 1567-5394
Roč. 103, June (2015), s. 103-110Number of pages 8 s. Publication form Print - P Language eng - English Country CH - Switzerland Keywords Electrical discharge ; Shock waves ; Tumor damage ; Necrosis ; Apoptosis Subject RIV BO - Biophysics R&D Projects ED2.1.00/03.0124 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UFP-V - RVO:61389021 ; UZFG-Y - RVO:67985904 UT WOS 000351977000017 EID SCOPUS 84923383214 DOI https://doi.org/10.1016/j.bioelechem.2014.08.019 Annotation Shock waves can cause significant cytotoxic effects in tumor cells and tissues both in vitro and in vivo.However,understanding the mechanisms of shock wave interaction with tissues is limited.We have studied in vivo effects of focused shock waves induced in the syngeneic sarcoma tumor model using the TUNEL assay,immunohistochemical detection of caspase-3 and hematoxylin-eosin staining.Shock waves were produced by a multichannel pulsed-electrohydraulic discharge generator with a cylindrical ceramic-coated electrode.In tumors treated with shock waves,a large area of damaged tissue was detected which was clearly differentiated from intact tissue. Localization and a cone-shaped region of tissue damage visualized by TUNEL reaction apparently correlated with the conical shape and direction of shock wave propagation determined by high-speed shadowgraphy.A strong TUNEL reaction of nuclei and nucleus fragments in tissue exposed to shock waves suggested apoptosis in this destroyed tumor area. Workplace Institute of Plasma Physics Contact Vladimíra Kebza, kebza@ipp.cas.cz, Tel.: 266 052 975 Year of Publishing 2016
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