0441045 - ÚFP 2016 RIV CH eng J - Journal Article
Lukeš, Petr - Zeman, J. - Horák, Vratislav - Hoffer, Petr - Poučková, P. - Holubová, Monika - Hosseini, S.H.R. - Akiyama, H. - Šunka, Pavel - Beneš, J.
In vivo effects of focused shock waves on tumor tissue visualized by fluorescence staining techniques.
Bioelectrochemistry. Roč. 103, June (2015), s. 103-110. ISSN 1567-5394. E-ISSN 1878-562X
R&D Projects: GA MŠMT ED2.1.00/03.0124
Grant - others:Rada Programu interní podpory projektů mezinárodní spolupráce AV ČR(CZ) M100431203
Program: M
Institutional support: RVO:61389021 ; RVO:67985904
Keywords : Electrical discharge * Shock waves * Tumor damage * Necrosis * Apoptosis
Subject RIV: BO - Biophysics
Impact factor: 3.556, year: 2015
Result website:
http://dx.doi.org/10.1016/j.bioelechem.2014.08.019
DOI: https://doi.org/10.1016/j.bioelechem.2014.08.019

Shock waves can cause significant cytotoxic effects in tumor cells and tissues both in vitro and in vivo.However,understanding the mechanisms of shock wave interaction with tissues is limited.We have studied in vivo effects of focused shock waves induced in the syngeneic sarcoma tumor model using the TUNEL assay,immunohistochemical detection of caspase-3 and hematoxylin-eosin staining.Shock waves were produced by a multichannel pulsed-electrohydraulic discharge generator with a cylindrical ceramic-coated electrode.In tumors treated with shock waves,a large area of damaged tissue was detected which was clearly differentiated from intact tissue. Localization and a cone-shaped region of tissue damage visualized by TUNEL reaction apparently correlated with the conical shape and direction of shock wave propagation determined by high-speed shadowgraphy.A strong TUNEL reaction of nuclei and nucleus fragments in tissue exposed to shock waves suggested apoptosis in this destroyed tumor area.

Permanent Link: http://hdl.handle.net/11104/0244096