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Comparison of Fractionation Techniques of CO2 Extracts from Eucalyptus Globulus - Composition and Insecticidal Activity

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    SYSNO ASEP0440668
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleComparison of Fractionation Techniques of CO2 Extracts from Eucalyptus Globulus - Composition and Insecticidal Activity
    Author(s) Topiař, Martin (UCHP-M) RID, ORCID, SAI
    Sajfrtová, Marie (UCHP-M) RID, ORCID, SAI
    Pavela, R. (CZ)
    Machalová, Zdeňka (UCHP-M) RID
    Source TitleJournal of Supercritical Fluids. - : Elsevier - ISSN 0896-8446
    Roč. 97, FEB 2015 (2015), s. 202-210
    Number of pages9 s.
    Languageeng - English
    CountryNL - Netherlands
    Keywordssupercritical fractinacion ; eucalyptus globulus ; insecticidal activity
    Subject RIVCI - Industrial Chemistry, Chemical Engineering
    R&D ProjectsTA01010578 GA TA ČR - Technology Agency of the Czech Republic (TA ČR)
    Institutional supportUCHP-M - RVO:67985858
    UT WOS000348952800024
    EID SCOPUS84937192203
    DOI https://doi.org/10.1016/j.supflu.2014.12.002
    AnnotationSupercritical fluid extraction combined with two fractionation methods, sorption and series of separators, was applied on Eucalyptus globulus leaves in order to maximize the insecticidal activity of extracts against Leptinotarasa decemlineata larvae. The major compounds in the essential oil isolated with the yield 26.7 mg/g were 1,8-cineole (42.6 wt%), -pinene (10.7 wt%) and globulol (5.9 wt%). The concentration of total volatiles in the extracts varied from 2.8 to 59.5 wt%, depending on the extraction and fractionation conditions. The extract obtained at 50 °C and 9 MPa exhibited the highest acute toxicity with LD50 = 38 µg. ScCO2 extracts exhibited several times stronger insecticidal activity in terms of chronic toxicity, antifeedant activity and growth inhibition than essential oil. Increase in the concentration of volatiles in isolates resulted into increase of chronic toxicity and antifeedant activity while growth inhibition decreased.
    WorkplaceInstitute of Chemical Process Fundamentals
    ContactEva Jirsová, jirsova@icpf.cas.cz, Tel.: 220 390 227
    Year of Publishing2015
Number of the records: 1  

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