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Characterization of the HMA7 gene and transcriptomic analysis of candidate genes for copper tolerance in two Silene vulgaris ecotypes
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SYSNO ASEP 0440398 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Characterization of the HMA7 gene and transcriptomic analysis of candidate genes for copper tolerance in two Silene vulgaris ecotypes Author(s) Baloun, Jiří (BFU-R)
Nevrtalová, Eva (BFU-R)
Kováčová, Viera (BFU-R)
Hudzieczek, Vojtěch (BFU-R) ORCID
Čegan, Radim (BFU-R) RID, ORCID
Vyskot, Boris (BFU-R) RID, ORCID
Hobza, Roman (BFU-R) RID, ORCIDNumber of authors 7 Source Title Journal of Plant Physiology. - : Elsevier - ISSN 0176-1617
Roč. 171, č. 13 (2014), s. 1188-1196Number of pages 9 s. Publication form Print - P Language eng - English Country PT - Portugal Keywords Copper ; Genes coding ROS-eliminating and Cu-transporting proteins ; RNA-Seq database Subject RIV BO - Biophysics R&D Projects GBP501/12/G090 GA ČR - Czech Science Foundation (CSF) LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support BFU-R - RVO:68081707 UT WOS 000345629900011 DOI 10.1016/j.jplph.2014.04.014 Annotation Silene vulgaris possesses ecotype-specific tolerance to high levels of copper in the soil. Although this was reported a few decades ago, little is known about this trait on a molecular level. The aim of this study was to analyze the transcription response to elevated copper concentrations in two S. vulgaris ecotypes originating from copper-contrasting soil types - copper-tolerant Lubietova and copper-sensitive Stranska skala. To reveal if plants are transcriptionally affected, we first analyzed the HMA7 gene, a known key player in copper metabolism. Based on BAC library screening, we identified a BAC clone containing a SvHMA7 sequence with all the structural properties specific for plant copper-transporting ATPases. The functionality of the gene was tested using heterologous complementation in yeast mutants. Analyses of SvHMA7 transcription patterns showed that both ecotypes studied up-regulated SvHMA7 transcription after the copper treatment. Our data are supported by analysis of appropriate reference genes based on RNA-Seq databases. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2015
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