Effect of H- and O-termination of nanocrystalline diamond films on cell adhesion and osteogenic differentiation

Lišková, Jana; Babchenko, Oleg; Varga, Marián; Kromka, Alexander; Hadraba, Daniel; Švindrych, Zdeněk; Bačáková, Lucie

Number of the records: 1

Effect of H- and O-termination of nanocrystalline diamond films on cell adhesion and osteogenic differentiation

1.

SYSNO ASEP	0440278
Document Type	C - Proceedings Paper (int. conf.)
R&D Document Type	Conference Paper
Title	Effect of H- and O-termination of nanocrystalline diamond films on cell adhesion and osteogenic differentiation
Author(s)	Lišková, Jana (FGU-C)_{RID, ORCID} Babchenko, Oleg (FZU-D)_{RID, ORCID} Varga, Marián (FZU-D)_{RID, ORCID} Kromka, Alexander (FZU-D)_{RID, ORCID, SAI} Hadraba, Daniel (FGU-C)_{RID, ORCID, SAI} Švindrych, Zdeněk (FGU-C) Bačáková, Lucie (FGU-C)_{RID, ORCID}
Source Title	NANOCON 2014 Conference Proceedings. - Ostrava : Tanger, 2014 - ISBN 978-80-87294-55-0
Number of pages	7 s.
Publication form	Medium - C
Action	International Conference NANOCON /6./
Event date	05.11.2014-07.11.2014
VEvent location	Brno
Country	CZ - Czech Republic
Event type	WRD
Language	eng - English
Country	CZ - Czech Republic
Keywords	nanocrystalline diamond film ; osteoblast ; MSC ; type I collagen ; paxillin
Subject RIV	BM - Solid Matter Physics ; Magnetism
R&D Projects	GA14-04790S GA ČR - Czech Science Foundation (CSF)
	EE2.3.30.0025 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	FZU-D - RVO:68378271 ; FGU-C - RVO:67985823
UT WOS	000350636300112
EID SCOPUS	85006008612
Annotation	In order to control the hydrophobic or hydrophilic character, the NCD film surfaces were grafted by hydrogen atoms (H-termination) or by oxygen atoms (O-termination). Human mesenchymal stem cells and primary human osteoblasts were used for biological studies on H- and O-terminated NCD films. We found that cells cultivated on O-terminated NCD films exhibit better adhesion than H-terminated NCD films. In addition, the expression of osteogenic cell markers collagen and alkaline phosphatase analysed by Realtime PCR and immunostaining was higher on O-terminated films.
Workplace	Institute of Physics
Contact	Kristina Potocká, potocka@fzu.cz, Tel.: 220 318 579
Year of Publishing	2015

Number of the records: 1