Organometallic Half-Sandwich Iridium Anticancer Complexes

0439813 - BFÚ 2015 RIV US eng J - Journal Article
Liu, Z. - Habtemariam, A. - Pizarro, A.M. - Fletcher, S.A. - Kisová, Anna - Vrána, Oldřich - Salassa, L. - Bruijnincx, P.C.A. - Clarkson, G.J. - Brabec, Viktor - Sadler, P. J.
Organometallic Half-Sandwich Iridium Anticancer Complexes.
Journal of Medicinal Chemistry. Roč. 54, č. 8 (2011), 3011-3026. ISSN 0022-2623. E-ISSN 1520-4804
R&D Projects: GA ČR(CZ) GPP303/11/P047; GA ČR(CZ) GAP301/10/0598
Keywords : RUTHENIUM(II) ARENE COMPLEXES * CANCER-CELL CYTOTOXICITY * DNA-BINDING PROPERTIES
Subject RIV: BO - Biophysics
Impact factor: 5.248, year: 2011

The low-spin 5d(6) Ir-III organometallic half-sandwich complexes [(eta(5)-Cp-x)Ir(XY)Cl](0/+), Cp-x = Cp*, tetramethyl(phenyl, cyclopentadienyl (Cp-xph), or tetramethyl(biphenyl)-cyclopertadienyl (Cp-xbiph), XY = 1,10-phenanthroline (4-6), 2,2'-bipyridine (7-9), ethylenediamine (10 and 11), or picolinate (12-14), hydrolyze rapidly. Complexes with N,N-chelating ligands readily form adducts with 9-ethylguanine but not 9-ethyladenine; picolinate complexes bind to both purines. Cytotoxic potency toward A2780 human ovarian cancer cells increases with phenyl substitution on Cp*: Cp-xbiph > Cp-xph > Cp*; Cp-xbiph complexes 6 and 9 have submicromolar activity. Guanine residues are preferential binding sites for 4-6 on plasmid DNA. Hydrophobicity (log P), cell and nucleus accumulation of Ir correlate with cytotoxicity, 6 > 5 > 4, they:distribute similarly within cells. The ability to displace DNA intercalator ethidium bromide from DNA correlates with cytotoxicity and viscosity of Ir DNA adducts. The hydrophobicity and intercalative ability of Cp-xph and Cp-xbiph male a major contribution to the anticancer potency of their Ir-III complexes.
Permanent Link: http://hdl.handle.net/11104/0243028