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Phytoplasmas and phytoplasma disease management: how to reduce their economic impact
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SYSNO ASEP 0439207 Document Type M - Monograph Chapter R&D Document Type Monograph Chapter Title Phytoplasma infections in Rhododendron hybridum Author(s) Přibylová, Jaroslava (BC-A) RID, ORCID
Špak, Josef (BC-A) RID, ORCID
Fránová, Jana (BC-A) RID, ORCIDSource Title Phytoplasmas and phytoplasma disease management: how to reduce their economic impact, The phytoplasmas and phytoplasma vectors in COST FA0807 Countries. - Bologna : IPWG - International Phytoplasmologist Working Group, 2014 / Bertaccini Assunta - ISBN 978-88-909922-0-9 Pages s. 137-142 Number of pages 6 s. Number of pages 287 Publication form Print - P Language eng - English Country IT - Italy Keywords Electron microscopy ; PCR ; phytoplasma ; sequencing Subject RIV EE - Microbiology, Virology R&D Projects LD12074 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support BC-A - RVO:60077344 Annotation The survey of the health status of azaleas and rhododendrons on production areas in the Czech Republic started in 2006. Symptoms of unknown aetiology on Rhododendron hybridum cultivar Cunningham’s White were observed in 2010. The infected plant had malformed leaves, with irregular shaped edges, mosaic, leaf tip necrosis and multiple axillary shoots with smaller leaves. Transmission electron microscopy showed phytoplasma-like bodies in phloem cells of the symptomatic plant. Phytoplasma presence was confirmed by polymerase chain reaction using phytoplasma-specific, universal and group specific primer pairs. Restriction fragment length polymorphism analysis of 16S rDNA enabled classification of the detected phytoplasma in the aster yellows subgroup 16SrI-C. Sequence analysis of the 16S-23S ribosomal operon of the amplified phytoplasma from the infected rhododendron plant (1,724 bp) confirmed the closest relationship with the Czech Echinacea purpurea phyllody phytoplasma. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2015
Number of the records: 1