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Enzymatic Sensor of Biogenic Amines with Optical Oxygen Transducer
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SYSNO ASEP 0439186 Document Type C - Proceedings Paper (int. conf.) R&D Document Type The record was not marked in the RIV Title Enzymatic Sensor of Biogenic Amines with Optical Oxygen Transducer Author(s) Maixnerová, Lucie (UCHP-M) RID
Horvitz, Alexandar (UCHP-M)
Kuncová, Gabriela (UCHP-M) RID, SAI
Přibyl, M. (CZ)
Šebela, M. (CZ)
Koštejn, Martin (UCHP-M) RID, SAI, ORCIDSource Title Book of Abstracts. - Brno : Masarykova Universita, 2014 - ISBN 978-80-210-7159-9 Pages s. 173 Number of pages 1 s. Publication form Print - P Action CEITEC Annual Conference Event date 21.10.2014-24.10.2014 VEvent location Brno Country CZ - Czech Republic Event type WRD Language eng - English Country CZ - Czech Republic Keywords optical enzymatic biosensor ; biogenic amines ; mathematical model Subject RIV CB - Analytical Chemistry, Separation R&D Projects TA03010544 GA TA ČR - Technology Agency of the Czech Republic (TA ČR) Institutional support UCHP-M - RVO:67985858 Annotation The biogenic amines (BA) are formed by the decarboxylation of amino acids. Because of their impact on human health, they have been used as markers for assessing the freshness and quality of a wide variety of protein-containing products. We studied biosensor for BA with an optical oxygen transducer that contained a diamine oxidase from pea and ruthenium complex. Both active components were incorporated in chemically stable polymer to form sensitive films thickness of 100 - 200 μm. With aim to optimize a composition and manufacturing of the biosensor we developer a mathematical model of processes inside the sensing film. The developed mathematical model revealed qualitative dependencies of the biosensor behavior (sensitivity, response time and dynamic range) on the (i) thickness of the sensitive layer, (ii) concentrations of substrates in the solution, (iii) enzyme activity in the layer and (iv) layer permeability for the substrates. Workplace Institute of Chemical Process Fundamentals Contact Eva Jirsová, jirsova@icpf.cas.cz, Tel.: 220 390 227 Year of Publishing 2015
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