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Impact of cadmium on the level of hepatic metallothioneins, essential elements, and selected enzymes in the experimental rat model
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SYSNO ASEP 0438976 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Impact of cadmium on the level of hepatic metallothioneins, essential elements, and selected enzymes in the experimental rat model Author(s) Zídková, J. (CZ)
Melčová, M. (CZ)
Bartošová, K. (CZ)
Šestáková, Ivana (UFCH-W) RID
Zídek, Václav (FGU-C) RID
Száková, J. (CZ)
Miholová, D. (CZ)
Tlustoš, P. (CZ)Source Title Czech Journal of Animal Science. - : Czech Academy of Agricultural Sciences - ISSN 1212-1819
Roč. 59, č. 12 (2014), s. 548-556Number of pages 9 s. Language eng - English Country CZ - Czech Republic Keywords metal intoxication ; liver ; blood plasma Subject RIV CF - Physical ; Theoretical Chemistry R&D Projects IAA400400806 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) GA13-04580S GA ČR - Czech Science Foundation (CSF) Institutional support UFCH-W - RVO:61388955 ; FGU-C - RVO:67985823 UT WOS 000346482200002 EID SCOPUS 84913588103 Annotation The response of different strains of laboratory rats (Rattus norvegicus L.) on both acute (via intraperitoneal injection) and chronic (via drinking water and/or diet) cadmium intoxication was investigated in the model study. The rat strains Long Evans (LE), Spontaneously hypertensive rat (SHR), and Brown Norway (BN) were tested and compared, and total Cd levels and metallothionein (MT) concentrations were determined in the liver of experimental animals. The liver MT concentrations were determined by using adsorptive chronopotentiometry and modified Brdicka reaction and were significantly correlated (r = 0.965) with the total liver Cd content. Moreover, the Cd application resulted in increasing zinc liver contents confirming intensive MT synthesis in the rat liver. In the blood plasma, specific enzymatic activity of glutathione reductase (GR) and glutathione-S-transferase (GST) was determined suggesting increasing activity of GR with the amount of applied Cd for all three strains, whereas ambiguous results have been found for the activity of GST. Therefore, MT concentrations seemed to be more sensitive indicators of the Cd intoxication compared to the assessment of the specific enzymatic activity. Workplace J. Heyrovsky Institute of Physical Chemistry Contact Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Year of Publishing 2015
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