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Enzymatic Sensor of Putrescine with Optical Oxygen Transducer - Mathematical Model of Responses of Sensitive Layer.
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SYSNO ASEP 0438314 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Enzymatic Sensor of Putrescine with Optical Oxygen Transducer - Mathematical Model of Responses of Sensitive Layer. Author(s) Maixnerová, Lucie (UCHP-M) RID
Horvitz, Alexandar (UCHP-M)
Kuncová, Gabriela (UCHP-M) RID, SAI
Přibyl, M. (CZ)
Šebela, M. (CZ)
Koštejn, Martin (UCHP-M) RID, SAI, ORCIDSource Title Chemical Papers. - : Springer - ISSN 0366-6352
Roč. 69, č. 1 (2015), s. 158-166Number of pages 9 s. Language eng - English Country SK - Slovakia Keywords enzymatic sensor ; putrescine ; optical oxygen transducer Subject RIV CE - Biochemistry R&D Projects TA03010548 GA TA ČR - Technology Agency of the Czech Republic (TA ČR) LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UCHP-M - RVO:67985858 UT WOS 000350130100014 EID SCOPUS 84916919884 DOI 10.1515/chempap-2015-0041 Annotation A biosensor for putrescine containing a sensing layer with an optical oxygen probe based on ruthenium complex and the enzyme diamine oxidase from pea is described. The diamine oxidase was pre-immobilised on broken micro-beads modified with a ferrofluid. The pre-immobilised enzyme and ruthenium complex were both incorporated into the UV-cured inorganic–organic hybrid polymer ORMOCER and deposited on a lens to form a sensitive layer of 210 μm in thickness. The sensitivity to the putrescine concentration determined under air saturation was between 3.50 μs L mmol-1 and 4.50 μs L mmol-1 in a hundred experiments conducted intermittently over a one year period. With the oxygen concentration increasing from 10 % to 100 % of DO (dissolved oxygen), the biosensor sensitivity decreased from 6.87 μs L mmol-1 to 0.70 μs L mmol-1 and its dynamic range increased from 0.10 mmol L-1 to 1.75 mmol L-1. To estimate the behaviour of the putrescine sensor in parametric space, a mathematical model of the reaction–transport processes inside the sensing layer was developed. The model revealed the qualitative relations between the sensor analytical features, the characteristics of the sensitive layer and concentrations of substrates. The results of the mathematical modelling may serve as guidelines in the design of optodes for specific applications. Workplace Institute of Chemical Process Fundamentals Contact Eva Jirsová, jirsova@icpf.cas.cz, Tel.: 220 390 227 Year of Publishing 2015
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