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Application of Fӧrster resonance energy transfer (FRET) for a detection of DNA mutations
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SYSNO ASEP 0437861 Document Type A - Abstract R&D Document Type The record was not marked in the RIV R&D Document Type Není vybrán druh dokumentu Title Application of Fӧrster resonance energy transfer (FRET) for a detection of DNA mutations Author(s) Datinská, Vladimíra (UIACH-O)
Klepárník, Karel (UIACH-O) RID, ORCID
Belšánová, Barbora (UIACH-O)
Minárik, M. (CZ)
Foret, František (UIACH-O) RID, ORCIDNumber of authors 5 Source Title ITP & LACE 2014. Book of Abstracts. - : Grupo VLS Print Solution, 2014 / Guzman N. A. ; Taveres M. F. M.
S. 93-93Number of pages 1 s. Publication form Online - E Action International Symposium on Electro- and Liquid Phase-Separation Techniques /21./ and Latin-American Symposium on Biotechnology, Biomedical, Biopharmaceutical, and Industrial Applications of Capillary Electrophoresis and Microchip Technology /21./ Event date 04.10.2014-08.10.2014 VEvent location Natal Country BR - Brazil Event type WRD Language eng - English Country BR - Brazil Keywords quantum dots ; Förster resonance energy transfer ; CE-LIF Subject RIV CB - Analytical Chemistry, Separation R&D Projects GA14-28254S GA ČR - Czech Science Foundation (CSF) TA02010672 GA TA ČR - Technology Agency of the Czech Republic (TA ČR) Institutional support UIACH-O - RVO:68081715 Annotation We have synthesized a probe consisting of CdTe QDs conjugated with oligonucleotide to be used as a donor. Conjugation reaction between carboxylated QDs and aminated oligonucleotides has been performed via zero-length cross-linkers. Sample consisting of clinically-relevant PCR amplicons labelled with ROX (6-carboxyrhodamine) served as acceptor. Upon mutation-specific sample-probe hybridization an increase in fluorescence intensity at 610 nm (the emission spectra maximum of ROX) confirmed a proper function of FRET at a presence of detected mutation. The limit of detection evaluated for PCR amplified fragments of the sample is 4x10-9 M. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2015
Number of the records: 1