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Sensitive detection of interferon-gamma with engineered proteins and surface plasmon resonance biosensor
- 1.0437424 - ÚFE 2015 RIV NL eng C - Conference Paper (international conference)
Šípová, Hana - Ševců, Veronika - Kuchař, Milan - Ahmad, Jawid Nazir - Mikulecký, Pavel - Šebo, Peter - Malý, Petr - Homola, Jiří
Sensitive detection of interferon-gamma with engineered proteins and surface plasmon resonance biosensor.
Procedia Engineering. Vol. 25. Amsterdam: Elsevier, 2011 - (Tsamis, C.; Kaltsas, G.), s. 940-943. ISBN 978-1-62748-581-4. ISSN 1877-7058.
[25th Eurosensors Conference. Athens (GR), 04.09.2011-07.09.2011]
R&D Projects: GA AV ČR KAN200670701; GA AV ČR KAN200520702; GA MŠMT OC09058
Institutional research plan: CEZ:AV0Z20670512; CEZ:AV0Z50520701; CEZ:AV0Z50200510
Keywords : Surface plasmon resonance * Biosensor * Interferon gamma
Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering; EI - Biotechnology ; Bionics (BTO-N); CE - Biochemistry (MBU-M)
We present a novel surface plasmon resonance (SPR) biosensor for detection of human interferon gamma (hIFNy), an important diagnostic marker for several infectious diseases. The biosensor employs engineered proteins derived from albumin binding domain (ABD) and selected with ribosome display to provide high affinity towards hIFNy. We demonstrate that the biosensor provides rapid, sensitive, and cost-effective platform for the detection of hIFNγ and allows its detection at nanomolar levels both in buffer and diluted human plasma
Permanent Link: http://hdl.handle.net/11104/0241007
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