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Possible mechanisms responsible for absence of a retrotransposon family on a plant Y chromosome
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SYSNO ASEP 0436683 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Possible mechanisms responsible for absence of a retrotransposon family on a plant Y chromosome Author(s) Kubát, Zdeněk (BFU-R) RID, ORCID
Žlůvová, Jitka (BFU-R) RID, ORCID
Vogel, Ivan (BFU-R)
Kováčová, Viera (BFU-R)
Čermák, Tomáš (BFU-R)
Čegan, Radim (BFU-R) RID, ORCID
Hobza, Roman (BFU-R) RID, ORCID
Vyskot, Boris (BFU-R) RID, ORCID
Kejnovský, Eduard (BFU-R) RID, ORCIDNumber of authors 9 Source Title New Phytologist - ISSN 0028-646X
Roč. 202, č. 2 (2014), s. 662-678Number of pages 17 s. Publication form Print - P Language eng - English Country GB - United Kingdom Keywords epigenetics ; genome size ; long terminal repeat Subject RIV BO - Biophysics R&D Projects GPP501/10/P483 GA ČR - Czech Science Foundation (CSF) GAP501/10/0102 GA ČR - Czech Science Foundation (CSF) ED1.1.00/02.0068 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EE2.3.20.0045 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support BFU-R - RVO:68081707 UT WOS 000333060500035 DOI 10.1111/nph.12669 Annotation Some transposable elements (TEs) show extraordinary variance in abundance along sex chromosomes but the mechanisms responsible for this variance are unknown. Here, we studied Ogre long terminal repeat (LTR) retrotransposons in Silene latifolia, a dioecious plant with evolutionarily young heteromorphic sex chromosomes. Ogre elements are ubiquitous in the S.latifolia genome but surprisingly absent on the Y chromosome. Bacterial artificial chromosome (BAC) library analysis and fluorescence in situ hybridization (FISH) were used to determine Ogre structure and chromosomal localization. Next generation sequencing (NGS) data were analysed to assess the transcription level and abundance of small RNAs. Methylation of Ogres was determined by bisulphite sequencing. Phylogenetic analysis was used to determine mobilization time and selection forces acting on Ogre elements. We characterized three Ogre families ubiquitous in the S.latifolia genome. One family is nearly absent on the Y chromosome despite all the families having similar structures and spreading mechanisms. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2015
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