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Mapping of single-copy genes by TSA-FISH in th codling moth, Cydia pomonella
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SYSNO ASEP 0436606 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Mapping of single-copy genes by TSA-FISH in th codling moth, Cydia pomonella Author(s) Carabajal Paladino, Leonela Z. (BC-A) RID
Nguyen, Petr (BC-A) RID, ORCID
Šíchová, Jindra (BC-A) RID
Marec, František (BC-A) RID, ORCIDNumber of authors 4 Source Title B M C Genetics - ISSN 1471-2156
Roč. 15, Supplement 2 (2014), S15Number of pages 8 s. Language eng - English Country GB - United Kingdom Keywords Cydia pomonella Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA14-22765S GA ČR - Czech Science Foundation (CSF) GP14-35819P GA ČR - Czech Science Foundation (CSF) EE2.3.30.0032 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support BC-A - RVO:60077344 UT WOS 000353980100016 DOI 10.1186/1471-2156-15-S2-S15 Annotation In this study, we adapted a FISH protocol with tyramide signal amplification (TSA-FISH) for detection of single-copy genes in Lepidoptera. We tested the protocol with probes prepared from partial sequences of Z-linked genes in the codling moth. Using a modified TSA-FISH protocol we successfully mapped a partial sequence of the Acetylcholinesterase 1 (Ace-1) gene to the Z chromosome and confirmed thus its Z-linkage. A subsequent combination of BAC-FISH with BAC probes containing anticipated neighbouring Z-linked genes and TSA-FISH with the Ace-1 probe allowed the integration of Ace-1 in the physical map of the codling moth Z chromosome. We also developed a two-colour TSA-FISH protocol which enabled us simultaneous localization of two Z-linked genes, Ace-1 and Notch, to the expected regions of the Z chromosome. Our results suggest that TSA-FISH represents a reliable technique for physical mapping of genes or localization of transgene cassettes on chromosomes of moths and butterflies. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2015 Electronic address http://www.biomedcentral.com/content/pdf/1471-2156-15-S2-S15.pdf
Number of the records: 1