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Current state and perspectives of penicillin G acylase-based biocatalyses
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SYSNO ASEP 0435793 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Current state and perspectives of penicillin G acylase-based biocatalyses Author(s) Marešová, Helena (MBU-M) RID, ORCID
Plačková, Martina (MBU-M) RID
Grulich, Michal (MBU-M) RID
Kyslík, Pavel (MBU-M) RID, ORCIDSource Title Applied Microbiology and Biotechnology. - : Springer - ISSN 0175-7598
Roč. 98, č. 7 (2014), s. 2867-2879Number of pages 11 s. Language eng - English Country DE - Germany Keywords Penicillin G acylase ; beta lactam biocatalysis ; Enantioselectivity Subject RIV EE - Microbiology, Virology Institutional support MBU-M - RVO:61388971 UT WOS 000334167900003 DOI https://doi.org/10.1007/s00253-013-5492-7 Annotation In the course of more than 60-year history, penicillin G acylase (PGA) gained a unique position among enzymes used by pharmaceutical industry for production of beta-lactam antibiotics. Kinetically controlled enzymatic syntheses of cephalosporins of novel generations in which PGA catalyzes coupling of activated acyl donor with nucleophile belong among the latest large-scale applications. Contrary to rather specific roles of other enzymes involved in beta-lactam biocatalyses, the PGA seems to have the greatest potential. On the laboratory scale, other applications with industrial potential were described, e.g., directed evolution of the enzyme to meet specific demands of industrial processes or its modification into the enzyme catalyzing reactions with novel substrates. The fact that beta-lactams represent the most important group of antibiotics comprising 65 % of the world antibiotic market explains such a tremendous and continuous interest in this enzyme. Indeed, the annual consumption of PGA has recently been estimated to range from 10 to 30 million tons. The application potential of the enzyme goes beyond the beta-lactam biocatalysis due to its enantioselectivity and promiscuity: the PGA can be used for the production of achiral and chiral compounds convenient for the preparation of synthons and active pharmaceutical ingrediences, respectively. These biocatalyses, however, still wait for large-scale application. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2015
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