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Depolarization affects lateral microdomain structure of yeast plasma membrane
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SYSNO ASEP 0435550 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Depolarization affects lateral microdomain structure of yeast plasma membrane Author(s) Herman, P. (CZ)
Večeř, J. (CZ)
Opekarová, Miroslava (UEM-P)
Veselá, Petra (UEM-P) RID, ORCID
Jančíková, I. (CZ)
Zahumenský, J. (CZ)
Malínský, Jan (UEM-P) RID, ORCIDNumber of authors 7 Source Title FEBS Journal - ISSN 1742-464X
Roč. 282, č. 3 (2015), s. 419-434Number of pages 16 s. Language eng - English Country GB - United Kingdom Keywords gel microdomains ; lipid order ; transmembrane potential Subject RIV EA - Cell Biology R&D Projects GAP205/12/0720 GA ČR - Czech Science Foundation (CSF) Institutional support UEM-P - RVO:68378041 UT WOS 000348901600001 EID SCOPUS 84921803051 DOI 10.1111/febs.13156 Annotation We report the transmembrane voltage-induced lateral reorganization of highly-ordered lipid microdomains in the plasma membrane of living yeast. Different depolarization mechanisms, including the application of ionophores, cell depolarization by an external electric field, and partial depolarization by protein-mediated ion transport or by compromised respiration efficiency, yielded the same results: a significant reduction in the amount of gel-like microdomains in the membrane. The data suggest that the voltage-induced reorganization of lateral membrane structure could play significant role in fast cellular response to acute stress conditions, as well as in other membrane microdomain-related regulatory mechanisms. Workplace Institute of Experimental Medicine Contact Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2016
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