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Phthalates Deregulate Cell Proliferation, but Not Neuroendocrine Transdifferentiation, in Human LNCaP Prostate Cancer Cell Model
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SYSNO ASEP 0435171 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Phthalates Deregulate Cell Proliferation, but Not Neuroendocrine Transdifferentiation, in Human LNCaP Prostate Cancer Cell Model Author(s) Hrubá, Eva (BFU-R)
Pernicová, Zuzana (BFU-R) RID
Palková, L. (CZ)
Souček, Karel (BFU-R) RID, ORCID
Vondráček, Jan (BFU-R) RID, ORCID
Machala, M. (CZ)Number of authors 6 Source Title Folia Biologica. - : Univerzita Karlova v Praze - ISSN 0015-5500
Roč. 60, č. 2014 (2014), s. 56-61Number of pages 6 s. Publication form Print - P Language eng - English Country CZ - Czech Republic Keywords phthalates ; prostate cancer cells ; cell cycle modulation Subject RIV BO - Biophysics R&D Projects EE2.3.30.0030 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED1.100/02/0123 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support BFU-R - RVO:68081707 UT WOS 000343275800009 Annotation Phthalate esters are ubiquitous environmental pollutants widely used as plasticizers, which have been shown to interfere with both endocrine regulation and development of reproductive organs. In the present study, we examined the impact of diethylhexyl phthalate (DEHP) and dibutyl phthalate (DBP) on the proliferation of androgen-sensitive human prostate carcinoma LNCaP cells and related events. The results showed that both compounds were able to inhibit cell cycle progression in a dose-dependent manner. However, only DEHP was found to weakly reduce androgen receptor (AR) protein levels after long-term exposure, while only DBP partially inhibited expression of the prostate-specific antigen (KLK3) gene, a model AR transcriptional target. This indicated that inhibition of cell proliferation was likely independent of any AR modulations. Both phthalates induced suppression of cell proliferation, but none of them affected the levels of markers associated with neuroendocrine transdifferentiation (NED) in LNCaP cells. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2015
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