Number of the records: 1  

Quantum Dots: Applications in Biology

  1. 1.
    SYSNO ASEP0433692
    Document TypeM - Monograph Chapter
    R&D Document TypeMonograph Chapter
    TitleAnalysis of quantum dots and their conjugates by capillary electrophoresis with detection of laser-induced luminescence
    Author(s) Klepárník, Karel (UIACH-O) RID, ORCID
    Datinská, Vladimíra (UIACH-O)
    Voráčová, Ivona (UIACH-O) RID, ORCID
    Lišková, Marcela (UIACH-O)
    Number of authors4
    Source TitleQuantum Dots: Applications in Biology. - Totowa : Humana Press Inc, 2014 / Fontes A. - ISSN 1064-3745 - ISBN 978-1-4939-1279-7
    Pagess. 33-54
    Number of pages21 s.
    Number of pages264
    Publication formPrint - P
    Languageeng - English
    CountryUS - United States
    Keywordscapillary electrophoresis ; quantum dots ; laser-induced fluorescence ; immuno assay
    Subject RIVCB - Analytical Chemistry, Separation
    R&D ProjectsGAP206/11/2377 GA ČR - Czech Science Foundation (CSF)
    GA14-28254S GA ČR - Czech Science Foundation (CSF)
    Institutional supportUIACH-O - RVO:68081715
    UT WOS000343087500004
    DOI10.1007/978-1-4939-1280-3_3
    AnnotationIn many bioanalytical applications, important molecules such as DNA, proteins, and antibodies are routinely conjugated with fluorescent tags to reach an extraordinary sensitivity of analyses. Semiconductor nanoparticles, quantum dots, have already proved to be suitable components of highly luminescent tags, probes, and sensors with a broad applicability in analytical chemistry. Quantum dots provide high extinction coefficients together with a wide range of excitation wavelengths, size- and composition-tunable emissions, narrow and symmetric emission spectra, good quantum yields, relatively long size-dependent luminescence lifetime, and practically no photobleaching. Most of these properties are superior when compared with conventional organic fluorescent dyes. In this chapter, optimized procedures for the preparation of water-dispersed cadmium telluride (CdTe) quantum dots, conjugating reactions with antibodies, DNA, and macrocycles as well as their analyses by capillary electrophoresis are described. The potential of capillary electrophoresis for fast analyses of nanoparticles, their conjugates with antibodies, and immunocomplexes with targeted antigens is demonstrated on examples.
    WorkplaceInstitute of Analytical Chemistry
    ContactIveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234
    Year of Publishing2015
Number of the records: 1  

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