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Quantum Dots: Applications in Biology
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SYSNO ASEP 0433692 Document Type M - Monograph Chapter R&D Document Type Monograph Chapter Title Analysis of quantum dots and their conjugates by capillary electrophoresis with detection of laser-induced luminescence Author(s) Klepárník, Karel (UIACH-O) RID, ORCID
Datinská, Vladimíra (UIACH-O)
Voráčová, Ivona (UIACH-O) RID, ORCID
Lišková, Marcela (UIACH-O)Number of authors 4 Source Title Quantum Dots: Applications in Biology. - Totowa : Humana Press Inc, 2014 / Fontes A. - ISSN 1064-3745 - ISBN 978-1-4939-1279-7 Pages s. 33-54 Number of pages 21 s. Number of pages 264 Publication form Print - P Language eng - English Country US - United States Keywords capillary electrophoresis ; quantum dots ; laser-induced fluorescence ; immuno assay Subject RIV CB - Analytical Chemistry, Separation R&D Projects GAP206/11/2377 GA ČR - Czech Science Foundation (CSF) GA14-28254S GA ČR - Czech Science Foundation (CSF) Institutional support UIACH-O - RVO:68081715 UT WOS 000343087500004 DOI 10.1007/978-1-4939-1280-3_3 Annotation In many bioanalytical applications, important molecules such as DNA, proteins, and antibodies are routinely conjugated with fluorescent tags to reach an extraordinary sensitivity of analyses. Semiconductor nanoparticles, quantum dots, have already proved to be suitable components of highly luminescent tags, probes, and sensors with a broad applicability in analytical chemistry. Quantum dots provide high extinction coefficients together with a wide range of excitation wavelengths, size- and composition-tunable emissions, narrow and symmetric emission spectra, good quantum yields, relatively long size-dependent luminescence lifetime, and practically no photobleaching. Most of these properties are superior when compared with conventional organic fluorescent dyes. In this chapter, optimized procedures for the preparation of water-dispersed cadmium telluride (CdTe) quantum dots, conjugating reactions with antibodies, DNA, and macrocycles as well as their analyses by capillary electrophoresis are described. The potential of capillary electrophoresis for fast analyses of nanoparticles, their conjugates with antibodies, and immunocomplexes with targeted antigens is demonstrated on examples. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2015
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