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Multiple, concentration-dependent effects of sucrose, auxins and cytokinins in explant cultures of kale and tobacco
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SYSNO ASEP 0432726 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Multiple, concentration-dependent effects of sucrose, auxins and cytokinins in explant cultures of kale and tobacco Author(s) Luštinec, Jiří (UEB-Q)
Cvrčková, F. (CZ)
Čížková, Jana (UEB-Q) RID, ORCID
Doležel, Jaroslav (UEB-Q) RID, ORCID
Kamínek, Miroslav (UEB-Q) RID, ORCID
Žárský, Viktor (UEB-Q) RID, ORCIDSource Title Acta Physiologiae Plantarum. - : Springer - ISSN 0137-5881
Roč. 36, č. 8 (2014), s. 1981-1991Number of pages 11 s. Language eng - English Country PL - Poland Keywords Brassica oleracea ; Nicotiana tabacum ; Absorption Subject RIV ED - Physiology R&D Projects LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEB-Q - RVO:61389030 UT WOS 000339817600003 DOI 10.1007/s11738-014-1573-1 Annotation We describe complex multiple concentration dependencies for the response of isolated pith tissues to plant biologically active substances. Kale and tobacco stem pith explants were cultured on agar media containing combinations of sucrose, cytokinin [kinetin or benzyladenine (BA)] and auxin [indole-3-acetic acid (IAA) or naphthaleneacetic acid (NAA)]. Absorption of these components by explants and their effects on explant mass, contents of soluble proteins, starch and sugars, and activity of ADP-glucose pyrophosphorylase (AGPase) were studied in relation to their concentration. Up to ten pronounced statistically significant maxima (peaks or waves) were repeatedly detected in the dose-response curves over a concentration range of several logarithmic orders. Slight maxima were observed in the corresponding absorption curves. Pronounced maxima of sucrose absorption were induced by IAA and BA, and those of NAA absorption were induced by sucrose. Both types of multiple maxima (in dose-response and absorption curves) may be due to changes in concentration of intracellular solutes (sugars, auxins and cytokinins), thereby affecting metabolic processes that act as sinks for external solutes and elicit feedback appearance of maxima in absorption curves. Good correspondence between external concentrations at which maxima of different compared curves occur in addition to statistical significance of individual maxima and repeatability of experimental results supports the conclusion that the multiple maxima exhibited are genuine. We consider it possibile that the multiple maxima are associated with endopolyploidy or mixoploidy and/or epigenomic diversity of pith cells that show different sensitivities to biologically active solutes. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2015
Number of the records: 1