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Establishment of Myotis myotis cell lines - model for investigation of host-pathogen interaction in a natural host for emerging viruses

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    SYSNO ASEP0432712
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleEstablishment of Myotis myotis cell lines - model for investigation of host-pathogen interaction in a natural host for emerging viruses
    Author(s) He, X. (DE)
    Korytář, T. (DE)
    Zhu, Y. (DE)
    Pikula, J. (CZ)
    Banďouchová, H. (CZ)
    Zukal, Jan (UBO-W) RID, ORCID, SAI
    Köllner, B. (DE)
    Number of authors7
    Source TitlePLoS ONE. - : Public Library of Science - ISSN 1932-6203
    Roč. 9, č. 10 (2014), e109795
    Number of pages10 s.
    Languageeng - English
    CountryUS - United States
    Keywordsbats ; emerging viruses
    Subject RIVEG - Zoology
    Institutional supportUBO-W - RVO:68081766
    UT WOS000345204000096
    EID SCOPUS84907822161
    DOI10.1371/journal.pone.0109795
    AnnotationBats are found to be the natural reservoirs for many emerging viruses. Due to the strict protection of European bat species, immortalized cell lines are the only alternative to investigate the innate anti-virus immune mechanisms. Here, we report about the establishment and functional characterization of Myotis myotis derived cell lines from different tissues: brain (MmBr), tonsil (MmTo), peritoneal cavity (MmPca), nasal epithelium (MmNep) and nervus olfactorius (MmNol) after immortalization by SV 40 large T antigen. The usefulness of these cell lines to study antiviral responses has been confirmed by analysis of their susceptibility to lyssavirus infection and the mRNA patterns of immune-relevant genes after poly I:C stimulation. Performed experiments indicated varying susceptibility to lyssavirus infection with MmBr being considerably less susceptible than the other cell lines. Further investigation demonstrated a strong activation of interferon mediated antiviral response in MmBr contributing to its resistance. The pattern recognition receptors: RIG-I and MDA5 were highly upregulated during rabies virus infection in MmBr, suggesting their involvement in promotion of antiviral responses. The presence of CD14 and CD68 in MmBr suggested MmBr cells are microglia-like cells which play a key role in host defense against infections in the central nervous system (CNS). Thus the expression pattern of MmBr combined with the observed limitation of lyssavirus replication underpin a protective mechanism of the CNS controlling the lyssavirus infection. Overall, the established cell lines are important tools to analyze antiviral innate immunity in M. myotis against neurotropic virus infections and present a valuable tool for a broad spectrum of future investigations in cellular biology of M. myotis.
    WorkplaceInstitute of Vertebrate Biology
    ContactHana Slabáková, slabakova@ivb.cz, Tel.: 543 422 524
    Year of Publishing2015
Number of the records: 1  

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