Plant Cell Morphogenesis: Methods and Protocols

0432111 - ÚEB 2015 RIV US eng M - Monography Chapter
Rosero, A. - Žárský, Viktor - Cvrčková, F.
Visualizing and Quantifying the In Vivo Structure and Dynamics of the Arabidopsis Cortical Cytoskeleton Using CLSM and VAEM.
Plant Cell Morphogenesis: Methods and Protocols. Vol. 1080. New York: Humana Press, 2014 - (Žárský, V.; Cvrčková, F.), s. 87-97. Methods in Molecular Biology. ISBN 978-1-62703-643-6
R&D Projects: GA ČR GAP305/10/0433
Institutional support: RVO:61389030
Keywords : Actin * Microtubules * Fluorescent proteins
Subject RIV: EB - Genetics ; Molecular Biology

The cortical microtubules, and to some extent also the actin meshwork, play a central role in the shaping of plant cells. Transgenic plants expressing fluorescent protein markers specifically tagging the two main cytoskeletal systems are available, allowing noninvasive in vivo studies. Advanced microscopy techniques, in particular confocal laser scanning microscopy (CLSM) and variable angle epifluorescence microscopy (VAEM), can be nowadays used for imaging the cortical cytoskeleton of living cells with unprecedented spatial and temporal resolution. With the aid of suitable computing techniques, quantitative information can be extracted from microscopic images and video sequences, providing insight into both architecture and dynamics of the cortical cytoskeleton.
Permanent Link: http://hdl.handle.net/11104/0236574