Study of DNA oligonucleotides interactions with ethidium bromide by partial-filling affinity capillary electrophoresis

0430047 - ÚOCHB 2015 CZ eng C - Conference Paper (international conference)
Růžička, Martin - Koval, Dušan - Kašička, Václav
Study of DNA oligonucleotides interactions with ethidium bromide by partial-filling affinity capillary electrophoresis.
Acta Universitatis Palackianae Olomucensis. Facultas Rerum Naturalium. Chemica 51S. Olomouc: Palacký University, 2014 - (Maier, V.; Ševčík, J.), s. 194-195. ISBN 978-80-244-3950-1. ISSN 0232-0061.
[Advances in Chromatography and Electrophoresis & Chiranal 2014. Olomouc (CZ), 10.02.2014-14.02.2014]
R&D Projects: GA ČR(CZ) GAP206/12/0453; GA ČR(CZ) GA13-17224S; GA ČR GA13-32974S
Institutional support: RVO:61388963
Keywords : affinity capillary electrophoresis * binding constant * oligonucleotide
Subject RIV: CB - Analytical Chemistry, Separation

A partial filling affinity capillary electrophoresis was developed and applied to investigation of non-covalent molecular interactions between double stranded DNA oligonucleotide (Dickerson dodecamer) and classical DNA intercalator ligand –ethidium bromide (EtBr). Binding constants of DNA EtBr complexes were determined from the dependence of migration time changes of DNA oligomer (applied as analyte) on the length of ligand zones introduced beforehand as plugs of various lengths in hydroxypropylcellulose coated fused silica capillary. Binding constants of DNA-EtBr complex were found to be in the range 4.2 x 103 – 1.5 104 L/mol.
Permanent Link: http://hdl.handle.net/11104/0235085