Changes in actin dynamics are involved in salicylic acid signaling pathway

0429540 - ÚEB 2015 RIV IE eng J - Journal Article
Matoušková, J. - Janda, M. - Fišer, R. - Šašek, Vladimír - Kocourková, Daniela - Burketová, Lenka - Dušková, J. - Martinec, Jan - Valentová, O.
Changes in actin dynamics are involved in salicylic acid signaling pathway.
Plant Science. Roč. 223, JUN 2014 (2014), s. 36-44. ISSN 0168-9452. E-ISSN 1873-2259
R&D Projects: GA ČR(CZ) GAP501/11/1654
Institutional support: RVO:61389030
Keywords : Actin dynamics * Salicylic acid * PR genes
Subject RIV: CE - Biochemistry
Impact factor: 3.607, year: 2014

Changes in actin cytoskeleton dynamics are one of the crucial players in many physiological as well as non-physiological processes in plant cells. Positioning of actin filament arrays is necessary for successful establishment of primary lines of defense toward pathogen attack, depolymerization leads very often to the enhanced susceptibility to the invading pathogen. On the other hand it was also shown that the disruption of actin cytoskeleton leads to the induction of defense response leading to the expression of PATHOGENESIS RELATED proteins (PR). In this study we show that pharmacological actin depolymerization leads to the specific induction of genes in salicylic acid pathway but not that involved in jasmonic acid signaling. Life imaging of leafs of Arabidopsis thaliana with GFP-tagged fimbrin (GFP-fABD2) treated with 1 mM salicylic acid revealed rapid disruption of actin filaments resembling the pattern viewed after treatment with 200 nM latrunculin B. The effect of salicylic acid on actin filament fragmentation was prevented by exogenous addition of phosphatidic acid, which binds to the capping protein and thus promotes actin polymerization. The quantitative evaluation of actin filament dynamics is also presented.
Permanent Link: http://hdl.handle.net/11104/0234631