0428917 - ÚIACH 2015 RIV DE eng J - Journal Article
Adamová, Eva - Lišková, Marcela - Matalová, E. - Klepárník, Karel
A miniaturized device for bioluminescence analysis of caspase-3/7 5 activity in a single apoptotic cell.
Analytical and Bioanalytical Chemistry. Roč. 406, č. 22 (2014), s. 5389-5394. ISSN 1618-2642. E-ISSN 1618-2650
R&D Projects: GA ČR(CZ) GA14-28254S
Institutional support: RVO:68081715
Keywords : apoptosis * bioluminescence * single-cell analysis
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 3.436, year: 2014 ; AIS: 0.91, rok: 2014
DOI: https://doi.org/10.1007/s00216-014-7949-7

Caspases are key enzymes activated during the 14 apoptotic machinery. Apoptosis as a way of programmed cell 15 death becomes deregulated in some pathologies including 16 cancer transformations, neurodegenerative, or autoimmune 17 diseases. Most of the methods available for the detection of 18 apoptosis and caspases provide qualitative information only or 19 quantification data as an average from cell populations or cell 20 lysates. Several reports point to the importance of more accu- 21 rate single-cell analyses in biomedical studies due to hetero- 22 geneity at tissue as well as cell level.
Permanent Link: http://hdl.handle.net/11104/0234138