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A simple and robust approach for genotyping in Musaceae
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SYSNO ASEP 0427110 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title A simple and robust approach for genotyping in Musaceae Author(s) Hřibová, Eva (UEB-Q) RID, ORCID
Christelová, Pavla (UEB-Q) RID, ORCID
Roux, N. (FR)
Doležel, Jaroslav (UEB-Q) RID, ORCIDSource Title Acta Horticulturae. - : International Society for Horticultural Science - ISSN 0567-7572
Roč. 986, April 2013 (2013), s. 241-246Number of pages 6 s. Language eng - English Country BE - Belgium Keywords fragment analysis ; genotyping platform ; microsatellite markers Subject RIV EB - Genetics ; Molecular Biology CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000332491200025 EID SCOPUS 84879231740 Annotation Banana and plantain (Musa spp.) are seed-sterile vegetatively propagated crops, which originated as intraspecific hybrids of Musa acuminate and interspecific hybrids between M. acuminate and Musa balbisiana. The rich genetic diversity of this crop is, however, endangered by diseases, adverse environmental conditions and changing farming practices. To set up an efficient and effective strategy for breeding improved banana cultivars and support the choice of crossing-parents, a solid understanding of the genetic diversity of available resources is needed, underlining the need for characterization and conservation of this genetic diversity. With the aim to provide a simple and robust approach for genotyping in Musa, we have developed an optimized genotyping platform using SSR markers. The genotyping system is based on 19 microsatellite loci, originally developed by CIRAD, which are scored using fluorescently labeled primers and high-throughput capillary electrophoresis separation with high resolution. The knowledge gained during the course of this study is used by the Musa Genotyping Centre, which serves the Musa research and breeding community by determining DNA ploidy and SSR profiles of unknown samples. The Centre operates on a cost-recovery basis and guarantees that all analyses are performed under strictly controlled conditions, enabling direct comparison of various accessions from different parts of the world. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2014 Electronic address http://www.actahort.org/books/986/986_25.htm
Number of the records: 1