Traceless bioresponsive shielding of adenovirus hexon with HPMA copolymers maintains transduction capacity in vitro and in vivo

0425747 - ÚMCH 2014 RIV US eng J - Journal Article
Prill, J.-M. - Šubr, Vladimír - Pasquarelli, N. - Engler, T. - Hoffmeister, A. - Kochanek, S. - Ulbrich, Karel - Kreppel, F.
Traceless bioresponsive shielding of adenovirus hexon with HPMA copolymers maintains transduction capacity in vitro and in vivo.
PLoS ONE. Roč. 9, č. 1 (2014), e82716_1-e82716_15. ISSN 1932-6203. E-ISSN 1932-6203
Grant - others:AV ČR(CZ) AP0802
Program: Akademická prémie - Praemium Academiae
Institutional support: RVO:61389013
Keywords : adenovirus * HPMA copolymers * bioresponsive shielding
Subject RIV: CD - Macromolecular Chemistry
Impact factor: 3.234, year: 2014

Capsid surface shielding of adenovirus vectors with synthetic polymers is an emerging technology to reduce unwanted interactions of the vector particles with cellular and non-cellular host components. While it has been shown that attachment of shielding polymers allows prevention of undesired interactions, it has become evident that a shield which is covalently attached to the vector surface can negatively affect gene transfer efficiency. Reasons are not only a limited receptor-binding ability of the shielded vectors but also a disturbance of intracellular trafficking processes, the latter depending on the interaction of the vector surface with the cellular transport machinery. A solution might be the development of bioresponsive shields that are stably maintained outside the host cell but released upon cell entry to allow for efficient gene delivery to the nucleus.
Permanent Link: http://hdl.handle.net/11104/0231620