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Simple power supply for power load controlled isoelectric focusing
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SYSNO ASEP 0425057 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Simple power supply for power load controlled isoelectric focusing Author(s) Duša, Filip (UIACH-O) RID, ORCID
Šlais, Karel (UIACH-O) RID, ORCIDNumber of authors 2 Source Title Electrophoresis. - : Wiley - ISSN 0173-0835
Roč. 35, č. 8 (2014), s. 1114-1117Number of pages 4 s. Language eng - English Country DE - Germany Keywords isoelectric focusing ; power supply ; voltage multiplier Subject RIV CB - Analytical Chemistry, Separation R&D Projects VG20102015023 GA MV - Ministry of Interior (MV) Institutional support UIACH-O - RVO:68081715 UT WOS 000334126600005 DOI https://doi.org/10.1002/elps.201300518 Annotation The power supply for IEF based on features of the Cockcroft-Walton voltage multiplier (CW VM) is described in this work. The article describes a design of the IEF power supply, its electric characteristics, and testing by IEF analysis. A circuit diagram of the power supply included two opposite charged branches (each consisting of four voltage doublers). The designed CW VM was powered by 230 V/50 Hz alternate current and it generated up to 5 kV and 90 mW at the output. Voltage and current characteristics of the power supply were measured by known load resistances in the range from 10 k to 1 G , which is a common resistance range for IEF strip geometry. Further, the power supply was tested by a separation of a model mixture of colored pI markers using a 175 × 3 × 0.5 mm focusing bed. Automatically limited power load enabled analysis of samples without previous optimization of the focusing voltage or electric current time courses according to sample composition. Moreover, the developed power supply did not produce any intrinsic heat and was easy to set up with cheap and commonly available parts. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2015 Electronic address http://hdl.handle.net/11104/0231022
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