0424665 - ÚIACH 2014 CZ eng D - Thesis
Duša, Filip
Multidimensional separation methods in analysis of complex biological mixtures.
Ústav analytické chemie AV ČR, v. v. i. Defended: Brno. 29.01.2014. - Brno: Masaryk university, Faculty of Science, 2014. 92 s.
R&D Projects: GA MŠMT(CZ) EE2.3.20.0182
Institutional support: RVO:68081715
Keywords : isoelectric focusing * liquid chromatography * two-dimensional separations * sample pretreatment * proteins
Subject RIV: CB - Analytical Chemistry, Separation

This work deals with two-dimensional separations of peptides and proteins from biological matrices. Theoretical introduction describes extraction of proteins from biological matrices, modes of isoelectric focusing (IEF) and liquid chromatography (LC) used for fractionation in two-dimensional separation schemes, and current papers dealing with combination of IEF and LC. In the results chapter there are four papers, which passed reviews and have been published in international scientific journals.
Permanent Link: http://hdl.handle.net/11104/0230708