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Novel human multiple myeloma cell line UHKT-893
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SYSNO ASEP 0423264 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Novel human multiple myeloma cell line UHKT-893 Author(s) Uherková, L. (CZ)
Vančurová, I. (CZ)
Vyhlídalová, I. (CZ)
Pleschnerová, M. (CZ)
Špička, I. (CZ)
Mihalová, R. (CZ)
Březinová, J. (CZ)
Hodný, Zdeněk (UMG-J) RID
Čermáková, K. (CZ)
Polanská, V. (CZ)
Marinov, I. (CZ)
Jedelský, P.L. (CZ)
Kuželová, K. (CZ)
Stöckbauer, P. (CZ)Source Title Leukemia Research. - : Elsevier - ISSN 0145-2126
Roč. 37, č. 3 (2013), s. 320-326Number of pages 7 s. Publication form Print - P Language eng - English Country GB - United Kingdom Keywords human myeloma cell line ; human multiple myeloma ; plasma cell ; IL-6 dependence ; immunoglobulin ; free light chain Subject RIV EB - Genetics ; Molecular Biology Institutional support UMG-J - RVO:68378050 UT WOS 000314871500017 DOI 10.1016/j.leukres.2012.12.004 Annotation We established and characterized a new IL-6 dependent multiple myeloma (MM) cell line UHKT-893 from the bone marrow of a relapsed 57-year-old woman. Using nephelometry, cells with plasma cell phenotype and morphology were found to secrete IgG and free kappa (κ)-light chain of immunoglobulin. κ-Light chain was also recognized intracellularly by flow cytometry and by mass spectrometry. VH4-39 region of IgVH genes was rearranged and somatically hypermutated. Cytogenetic analysis of cells revealed new chromosome abnormalities in all breakpoints unique in both MM patients and cell lines – t(1;6), t(1;11), t(5;15), t(5;21), +der(11;15) and der(16). IL-6 independent subline UHKT-893a was established by adaptation to descending IL-6 concentration, while the original cell line keeps on maintaining its IL-6 dependency. The cell line provides a suitable material for cellular and molecular studies of tumor abnormalities, with potentially unique mutagenic features of myeloma disease. It may be utilized for human hybridoma construction and vaccine development. Both IL-6 dependent and independent cell clones represent an important model for studies of myeloma cell growth and resistance emerging during targeted therapy. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2014
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