H-terminated diamond as optically transparent impedance sensor for realtime monitoring of cell growth

0423258 - FZÚ 2014 AT eng A - Abstract
Ižák, Tibor - Novotná, Katarína - Bačáková, Lucie - Rezek, Bohuslav - Kromka, Alexander
H-terminated diamond as optically transparent impedance sensor for realtime monitoring of cell growth.
International Winterschool on Electronic Properties of Novel Materials /27./. Kirchberg: Kirchberg, 2013 - (Machon, M.; Herziger, F.). s. 102-103
[International Winterschool on Electronic Properties of Novel Materials /27./. 02.03.2013-09.03.2013, Kirchberg]
R&D Projects: GA ČR(CZ) GBP108/12/G108
Institutional support: RVO:68378271 ; RVO:67985823
Keywords : transparent * spectroscopy * nanocrystalline diamond
Subject RIV: BM - Solid Matter Physics ; Magnetism

Here we introduce diamond-based impedance sensor as an innovative solution for a wide range of applications in biosensorics and biomedicine. In this study we used intrinsic nanocrystalline diamond (NCD) lm to realize fully optically transparent in-plane impedance sensor where the electrodes are realized by p-type surface conductive channel induced by hydrogen termination of diamond. Cultivation of human osteoblast-like MG 63 cells was monitored by dierent diamond-based sensors in wide frequency range (100 kHz - 10 Hz) for several days and compared to commercially available reference sensor. The growth of cells were analysed by fluorescence microscopy. The measured impedance curves correspond well to the cell life cycle (adhesion, growth and detachment from the growth substrate). The equivalent electrical circuit of the sensor system was evaluated from the Nyquist plots and discussed in article.
Permanent Link: http://hdl.handle.net/11104/0229330