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Utilization of micropreparative fast focusing by a new wide pH range electrolyte system based on bidirectional isotachophoresis
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SYSNO ASEP 0423163 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Utilization of micropreparative fast focusing by a new wide pH range electrolyte system based on bidirectional isotachophoresis Author(s) Vykydalová, Marie (UIACH-O)
Duša, Filip (UIACH-O) RID, ORCID
Horká, Marie (UIACH-O) RID, ORCID
Šlais, Karel (UIACH-O) RID, ORCIDNumber of authors 4 Source Title Chemické Listy. Roč. 107, Issue s3.. - Praha : Česká společnost chemická, 2013 - ISSN 0009-2770 Pages s. 471-472 Number of pages 3 s. Publication form Online - E Action CECE Junior 2013 Event date 12.11.2013-13.11.2013 VEvent location Brno Country CZ - Czech Republic Event type WRD Language eng - English Country CZ - Czech Republic Keywords bidirectional isotachophoresis ; electrolyte system ; preparative focusing Subject RIV CB - Analytical Chemistry, Separation R&D Projects VG20112015021 GA MV - Ministry of Interior (MV) Institutional support UIACH-O - RVO:68081715 UT WOS 000328730800076 Annotation We suggest the possibility of practical utilization of a new electrolyte system for fast preparative focusing in wide pH range based on bidirectional isotachophoresis. The focusing occurs on nonwoven fabric strip positioned in an open horizontal V-shaped trough. It is based on bidirectional ITP with multiple counter ions and spacers created from commercially available simple buffers. Milk spiked with ampicillin was used as a sample and high performance liquid chromatography was as a second dimension for analysis of fast preparative focusing fractions. Speed, easy fraction handling, and possibility of pre-concentration of analytes from a raw sample are the benefits of this technique. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2014
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