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Detailed kinetic analysis of the interaction between the FOXO4–DNA-binding domain and DNA
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SYSNO ASEP 0397654 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Detailed kinetic analysis of the interaction between the FOXO4–DNA-binding domain and DNA Author(s) Vácha, P. (CZ)
Zusková, Iva (FGU-C)
Bumba, Ladislav (MBU-M) RID, ORCID
Večeř, J. (CZ)
Obšilová, Veronika (FGU-C) RID, ORCID, SAI
Obšil, T. (CZ)Source Title Biophysical Chemistry. - : Elsevier - ISSN 0301-4622
Roč. 184, DEC 31 (2013), s. 68-78Number of pages 11 s. Language eng - English Country NL - Netherlands Keywords binding kinetics ; DNA-binding domain ; FOXO4 forkhead transcription factor Subject RIV BO - Biophysics Subject RIV - cooperation Institute of Microbiology - Biochemistry R&D Projects GAP207/11/0717 GA ČR - Czech Science Foundation (CSF) Institutional support FGU-C - RVO:67985823 ; MBU-M - RVO:61388971 UT WOS 000328443300010 EID SCOPUS 84885446761 DOI 10.1016/j.bpc.2013.09.002 Annotation In this work, the real-time kinetics of the interaction between the FOXO4-DNA binding domain (FOXO4-DBD) and the DNA was studied by using surface plasmon resonance (SPR). SPR analysis revealed that the interaction between FOXO4-DBD and the double stranded DNA containing either the insulin-responsive or the Daf-16 family member-binding element is preferably described by using a conformational change model which suggests a structural change of FOXO4-DBD upon binding to the DNA. Alanine scanning of amino acid residues engaged in polar contacts with the DNA showed that certain non-specific contacts with the DNA backbone are very important for both the binding affinity and the binding specificity of FOXO4-DBD Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2014
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