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Aster Yellows Subgroup 16SrI-C Phytoplasma in Rhododendron hybridum
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SYSNO ASEP 0395153 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Aster Yellows Subgroup 16SrI-C Phytoplasma in Rhododendron hybridum Author(s) Přibylová, Jaroslava (BC-A) RID, ORCID
Špak, Josef (BC-A) RID, ORCID
Fránová, Jana (BC-A) RID, ORCIDSource Title Journal of Phytopathology - Phytopathologische Zeitschrift. - : Wiley - ISSN 0931-1785
Roč. 161, 7-8 (2013), s. 590-593Number of pages 4 s. Publication form Print - P Language eng - English Country DE - Germany Keywords electron microscopy ; PCR ; phytoplasma Subject RIV EE - Microbiology, Virology R&D Projects LD12074 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support BC-A - RVO:60077344 UT WOS 000321507600019 DOI 10.1111/jph.12099 Annotation Symptoms of unknown aetiology on Rhododendron hybridum were observed in the Czech Republic in 2010. The infected plant had malformed leaves, with irregular shaped edges, mosaic, leaf tip necrosis and multiple axillary shoots with smaller leaves. Transmission electron microscopy showed phytoplasma-like bodies in phloem cells of the symptomatic plant. Phytoplasma presence was confirmed by PCR using phytoplasma-specific, universal and group-specific primer pairs. RFLP analysis of 16S rDNA enabled classification of the detected phytoplasma into the aster yellows subgroup I-C. Sequence analysis of the 16S-23S ribosomal operon of the amplified phytoplasma genome from the infected rhododendron plant (1724bp) confirmed the closest relationship with the Czech Echinacea purpurea phyllody phytoplasma. These data suggest Rhododendron hybridum is a new host for the aster yellows phytoplasma subgroup 16SrI-C in the Czech Republic and worldwide. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2014
Number of the records: 1