Number of the records: 1
Cross-talk between Tetraspanin CD9 and Transmembrane Adaptor Protein Non-T Cell Activation Linker (NTAL) in Mast Cell Activation and Chemotaxis
- 1.
SYSNO ASEP 0392777 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Cross-talk between Tetraspanin CD9 and Transmembrane Adaptor Protein Non-T Cell Activation Linker (NTAL) in Mast Cell Activation and Chemotaxis Author(s) Hálová, Ivana (UMG-J) RID, ORCID
Dráberová, Lubica (UMG-J) RID
Bambousková, Monika (UMG-J)
Machyna, Martin (UMG-J)
Stegurová, Lucie (UMG-J)
Smrž, Daniel (UMG-J)
Dráber, Petr (UMG-J) RIDSource Title Journal of Biological Chemistry. - : Elsevier - ISSN 0021-9258
Roč. 288, č. 14 (2013), s. 9801-9814Number of pages 14 s. Language eng - English Country US - United States Keywords mast cell ; chemotaxis ; Fc receptor Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA301/09/1826 GA ČR - Czech Science Foundation (CSF) GAP302/10/1759 GA ČR - Czech Science Foundation (CSF) GBP302/12/G101 GA ČR - Czech Science Foundation (CSF) GD204/09/H084 GA ČR - Czech Science Foundation (CSF) LD12073 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) TA01010436 GA TA ČR - Technology Agency of the Czech Republic (TA ČR) FR-TI3/067 GA MPO - Ministry of Industry and Trade (MPO) Institutional support UMG-J - RVO:68378050 UT WOS 000317114000021 DOI 10.1074/jbc.M112.449231 Annotation Chemotaxis, a process leading to movement of cells towards increasing concentrations of chemoattractants, is essential, among others, for recruitment of mast cells within target tissues where they play an important role in innate and adaptive immunity. In this study we prepared a new mAb specific for the tetraspanin CD9. Binding of the antibody to bone marrow derived mast cells (BMMCs) triggered activation events which included cell degranulation, Ca2+ response, dephosphorylation of ezrin/radixin/moesin (ERM) family proteins and potent tyrosine phosphorylation of the non-T cell activation linker (NTAL) but only weak phosphorylation of the linker for activation of T cells (LAT). As documented by electron microscopy of isolated plasma membrane sheets, CD9 colocalized with the high-affinity IgE receptor (FcεRI) and NTAL but not with LAT. Further tests showed that both anti-CD9 antibody and its F(ab)2 fragment inhibited mast cell chemotaxis towards antigen. Experiments with BMMCs deficient in NTAL and/or LAT revealed different roles of these two adaptors in antigen-driven chemotaxis. The combined data indicate that chemotaxis towards antigen is controlled in mast cells by a crosstalk among FcεRI, tetraspanin CD9, transmembrane adaptor proteins NTAL and LAT and cytoskeleton-regulatory proteins of the ERM family. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2014
Number of the records: 1