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Nuclear Import of Chromatin Remodeler Isw1 Is Mediated by Atypical Bipartite cNLS and Classical Import Pathway
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SYSNO ASEP 0392728 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Nuclear Import of Chromatin Remodeler Isw1 Is Mediated by Atypical Bipartite cNLS and Classical Import Pathway Author(s) Vašicová, Pavla (MBU-M) RID
Strádalová, Vendula (MBU-M)
Halada, Petr (MBU-M) RID, ORCID
Hašek, Jiří (MBU-M) RID, ORCID
Malcová-Janatová, Ivana (MBU-M) RIDSource Title Traffic. - : Wiley - ISSN 1398-9219
Roč. 14, č. 2 (2013), s. 176-193Number of pages 18 s. Language eng - English Country US - United States Keywords bipartite NLS ; extended linker ; importin-a Subject RIV CE - Biochemistry R&D Projects IAA5020409 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) LC545 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support MBU-M - RVO:61388971 UT WOS 000313129100005 DOI https://doi.org/10.1111/tra.12025 Annotation The protein Isw1 of Saccharomyces cerevisiae is an imitation-switch chromatin-remodeling factor. We studied the mechanisms of its nuclear import and found that the nuclear localization signal (NLS) mediating the transport of Isw1 into the nucleus is located at the end of the C-terminus of the protein (aa10791105). We show that it is an atypical bipartite signal with an unconventional linker of 19 aa (KRIR X19 KKAK) and the only nuclear targeting signal within the Isw1 molecule. The efficiency of Isw1 nuclear import was found to be modulated by changes to the amino acid composition in the vicinity of the KRIR motif, but not by the linker length. Live-cell imaging of various karyopherin mutants and in vitro binding assays of Isw1NLS to importin-a revealed that the nuclear translocation of Isw1 is mediated by the classical import pathway Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2014
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