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Transposon-mediated transgenesis, transgenic rescue, and tissue-specific gene expression in rodents and rabbits
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SYSNO ASEP 0392101 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Transposon-mediated transgenesis, transgenic rescue, and tissue-specific gene expression in rodents and rabbits Author(s) Katter, K. (AT)
Geurts, A.M. (US)
Hoffmann, O. (HU)
Mátés, L. (US)
Landa, Vladimír (FGU-C) RID
Hiripi, L. (HU)
Moreno, C. (US)
Lazar, J. (US)
Bashir, S. (DE)
Zídek, Václav (FGU-C) RID
Popova, E. (DE)
Jerchow, B. (DE)
Becker, K. (DE)
Deveraj, A. (DE)
Walter, A. (AT)
Grzybowski, M. (US)
Corbett, M. (US)
Rangel, A.F. (US)
Hodges, M.R. (US)
Bader, M. (US)
Ivics, Z. (DE)
Jacob, H. J. (US)
Pravenec, Michal (FGU-C) RID, ORCID
Bösze, Z. (HU)
Rülicke, T. (AT)
Izsvák, Z. (DE)Source Title FASEB Journal. - : Wiley - ISSN 0892-6638
Roč. 27, č. 3 (2013), s. 930-941Number of pages 12 s. Language eng - English Country US - United States Keywords transgenisis ; transposon Sleeping Beauty ; SB100X transposase Subject RIV EB - Genetics ; Molecular Biology R&D Projects GAP301/10/0290 GA ČR - Czech Science Foundation (CSF) LL1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) 7E10067 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LH12061 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support FGU-C - RVO:67985823 UT WOS 000315585200009 EID SCOPUS 84874610564 DOI https://doi.org/10.1096/fj.12-205526 Annotation Germline transgenesis is an important procedure for functional investigations of biological pathways as well as for animal biotechnology. We’ve established a simple, non-viral protocol in three important biomedical model organisms, mouse, rat and rabbit. The protocol is based on the Sleeping Beauty transposon system (SB100X), and promotes early transgene integrations Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2014
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