Structure of glutaminyl cyclase from Drosophila melanogaster in space group I4

0391838 - ÚMCH 2014 RIV GB eng J - Journal Article
Kolenko, Petr - Koch, B. - Rahfeld, J.-U. - Schilling, S. - Demuth, H.-U. - Stubbs, M. T.
Structure of glutaminyl cyclase from Drosophila melanogaster in space group I4.
Acta Crystallographica Section F-Structural Biology and Crystallization Communications. Roč. 69, č. 4 (2013), s. 358-361. ISSN 1744-3091. E-ISSN 2053-230X
R&D Projects: GA MŠMT EE2.3.30.0029
Institutional support: RVO:61389013
Keywords : glutaminyl cyclases * Drosophila melanogaster * soaking
Subject RIV: CE - Biochemistry
Impact factor: 0.568, year: 2013

The structure of ligand-free glutaminyl cyclase (QC) from Drosophila melanogaster (DmQC) has been determined in a novel crystal form. The protein crystallized in space group I4, with unit-cell parameters a = b = 122.3, c = 72.7 Å. The crystal diffracted to a resolution of 2 Å at the home source. The structure was solved by molecular replacement and was refined to an R factor of 0.169. DmQC exhibits a typical α/β-hydrolase fold. The electron density of three monosaccharides could be localized. The accessibility of the active site will facilitate structural studies of novel inhibitor-binding modes.
Permanent Link: http://hdl.handle.net/11104/0221172