De Novo Biosynthesis of Sexual Pheromone in the Labial Gland of Bumblebee Males

Žáček, Petr; Prchalová-Horňáková, Darina; Tykva, Richard; Kindl, Jiří; Vogel, H.; Svatoš, Aleš; Pichová, Iva; Valterová, Irena

doi:https://dx.doi.org/10.1002/cbic.201200684

Number of the records: 1

De Novo Biosynthesis of Sexual Pheromone in the Labial Gland of Bumblebee Males

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SYSNO ASEP	0391726
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	De Novo Biosynthesis of Sexual Pheromone in the Labial Gland of Bumblebee Males
Author(s)	Žáček, Petr (UOCHB-X)_RID Prchalová-Horňáková, Darina (UOCHB-X) Tykva, Richard (UOCHB-X) Kindl, Jiří (UOCHB-X)_RID Vogel, H. (DE) Svatoš, Aleš (UOCHB-X)_{ORCID, RID} Pichová, Iva (UOCHB-X)_{RID, ORCID} Valterová, Irena (UOCHB-X)_{RID, ORCID}
Number of authors	8
Source Title	Chembiochem. - : Wiley - ISSN 1439-4227 Roč. 14, č. 3 (2013), s. 361-371
Number of pages	11 s.
Language	eng - English
Country	DE - Germany
Keywords	labial gland ; Bombus lucorum ; Bombus terrestris ; fat bodies ; gene expression
Subject RIV	CE - Biochemistry
R&D Projects	GA203/09/1446 GA ČR - Czech Science Foundation (CSF)
Institutional support	UOCHB-X - RVO:61388963
UT WOS	000315491700012
EID SCOPUS	84873489090
DOI	10.1002/cbic.201200684
Annotation	De novo biosynthesis of male sex pheromone from two bumblebee species (Bombus terrestris and Bombus lucorum) was studied by using in vitro incubations of labial glands (LGs) with radioactive [1,2-C-14] acetate and deuterated [D-3]acetate. The labeled substrate was incorporated into several types of compounds, such as terpenic alcohols, fatty acids, esters, and hydrocarbons. A similar incubation of [1,2-C-14] acetate with fat bodies (FB) led to the formation of fatty acids, triacylglycerols (TAG), and hydrocarbons. To support the results from in vitro incubations, PCR analysis of fatty acid synthase (FAS) transcripts in LG and FB was performed. Relative quantification of FAS transcription levels revealed that the abundance of mRNA from the FAS gene is a function of the age of B. terrestris males. A comparison of the relative FAS mRNA gene transcription level in FB and LGs of B. terrestris and B. lucorum males proved that high biosynthetic activity takes place in the LGs of both species. Together, these results indicate that pheromone components are synthesized de novo in the LG.
Workplace	Institute of Organic Chemistry and Biochemistry
Contact	asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418
Year of Publishing	2014

Number of the records: 1