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Microfluidic devices for analysis and active optical sorting of individual cells
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SYSNO ASEP 0391348 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Microfluidic devices for analysis and active optical sorting of individual cells Author(s) Ježek, Jan (UPT-D) RID, ORCID, SAI
Pilát, Zdeněk (UPT-D) RID, SAI, ORCID
Šerý, Mojmír (UPT-D) RID, SAI
Kaňka, Jan (UPT-D) RID, SAI
Samek, Ota (UPT-D) RID, ORCID, SAI
Bernatová, Silvie (UPT-D) RID, SAI
Zemánek, Pavel (UPT-D) RID, SAI, ORCIDNumber of authors 7 Source Title Jemná mechanika a optika. - : Fyzikální ústav AV ČR, v. v. i. - ISSN 0447-6441
Roč. 58, č. 2 (2013), s. 55-59Number of pages 5 s. Publication form Print - P Language eng - English Country CZ - Czech Republic Keywords microfluidic ; cell sorting ; optical tweezers ; Raman spectroscopy Subject RIV EI - Biotechnology ; Bionics R&D Projects FR-TI1/433 GA MPO - Ministry of Industry and Trade (MPO) ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GAP205/11/1687 GA ČR - Czech Science Foundation (CSF) Institutional support UPT-D - RVO:68081731 Annotation We have constructed a device for active optical manipulation and Raman spectral analysis in a microfluidic environment. This system alllows effecient, nondestructive and contactless sorting of biological samples based on the Raman spectroscopie characteristics of individual living cells. The sorting platform used a combination of fast galvano-optics laser steering system and specially designed microfluidic chips. We used X shaped channels with two input and output ports as well as several other variants with different flow characteristics. The steerable trapping laser beam was designed to move the cell to the specified locations and confine the cell for the time period needed for the analysis. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2014
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