Intra-observer error of mouse long bone cross section digitization

0390442 - ÚBO 2013 RIV CZ eng J - Journal Article
Jindrová, A. - Tůma, J. - Sládek, Vladimír
Intra-observer error of mouse long bone cross section digitization.
Folia zoologica. Roč. 61, 3-4 (2012), s. 340-349. ISSN 0139-7893. E-ISSN 1573-1189
R&D Projects: GA ČR GA206/09/0589
Institutional support: RVO:68081766
Keywords : accuracy * cortical bone * CSG * digitization * tibia
Subject RIV: EG - Zoology
Impact factor: 0.494, year: 2012

Digitization of periosteal and endosteal contours of bone cross section is one of several methods used for calculating long bone cross-sectional geometry (CSG). In this study, invasively obtained bone histological samples were used to calculate intra-observer measurement error for CSG parameters. Intra-observer error was measured based upon repeated measurements of cross-sectional areas (total area [TA], cortical area [CA]) and moments of area (I-max, I-min, I-max/I-min, J) in B6CBA mice (n = 17). Cross sections were cut at 50 % of the biomechanical length of the left tibia and the samples were further processed for CSG and histological analysis. Intra-observer error was measured to estimate the accuracy of the digitization method. Accuracy of the tested digitization method was expressed by mean difference (MD), mean absolute difference (MAD), and limits of agreement (LA). The results confirm our assumption that intra-observer error decreases with the number of repeated measurement events. Thus, the error can be minimized by acquiring experience in the section digitizing. Our results also show that TA, CA and polar moment of area (J) are more susceptible to intra-observer error than are I-max, I-min and I-max/I-min.
Permanent Link: http://hdl.handle.net/11104/0219308