Electrochemical Sensing of Total Antioxidant Capacity and Polyphenol Content in Wine Samples Using Amperometry Online-Coupled with Microdialysis

0389674 - BFÚ 2013 RIV US eng J - Journal Article
Jakubec, P. - Bančíčová, M. - Halouzka, V. - Lojek, Antonín - Číž, Milan - Denev, P. - Cibiček, N. - Vacek, J. - Vostálová, J. - Ulrichová, J. - Hrbáč, J.
Electrochemical Sensing of Total Antioxidant Capacity and Polyphenol Content in Wine Samples Using Amperometry Online-Coupled with Microdialysis.
Journal of Agricultural and Food Chemistry. Roč. 60, č. 32 (2012), s. 7836-7843. ISSN 0021-8561. E-ISSN 1520-5118
Grant - others:GA ČR(CZ) GAP303/12/G163; GA MPO(CZ) FR-TI4/457; PrF(CZ) PrF_2011_025
Program: GA
Institutional research plan: CEZ:AV0Z50040702
Institutional support: RVO:68081707
Keywords : wine * antioxidant capacity * phenolics
Subject RIV: BO - Biophysics
Impact factor: 2.906, year: 2012

This work describes the method for total antioxidant capacity (TAC) and/or total content of phenolics (TCP) analysis in wines using microdialysis online-coupled with amperometric detection using a carbon microfiber working electrode. The system was tested on 10 selected wine samples, and the results were compared with total reactive antioxidant potential (TRAP), oxygen radical absorbance capacity (ORAC), and chemiluminescent determination of total antioxidant capacity (CL-TAC) methods using Trolox and catechin as standards. Microdialysis online-coupled with amperometric detection gives similar results to the widely used cyclic voltammetry methodology and closely correlates with ORAC and TRAP. The problem of electrode fouling is overcome by the introduction of an electrochemical cleaning step (1-2 min at the potential of 0 V vs Ag/AgCl). Such a procedure is sufficient to fully regenerate the electrode response for both red and white wine samples as well as catechin/Trolox standards
Permanent Link: http://hdl.handle.net/11104/0218540